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Genetic diversity of human sapovirus across the Americas

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dc.contributor.author Diez-Valcarce, Marta
dc.contributor.author Castro, Christina J.
dc.contributor.author Marine, Rachel L.
dc.contributor.author Halasa, Natasha
dc.contributor.author Mayta, Holger
dc.contributor.author Saito, Mayuko
dc.contributor.author Tsaknaridis, Laura
dc.contributor.author Pan, Chao-Yang
dc.contributor.author Bucardo, Filemon
dc.contributor.author Becker-Dreps, Sylvia
dc.contributor.author Lopez, Maria Renee
dc.contributor.author Magaña, Laura Cristal
dc.contributor.author Ng, Terry Fei Fan
dc.contributor.author Vinjé, Jan
dc.date.accessioned 2018-11-30T03:10:47Z
dc.date.available 2018-11-30T03:10:47Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4078
dc.description.abstract BACKGROUND: Sapoviruses are responsible for sporadic and epidemic acute gastroenteritis worldwide. Sapovirus typing protocols have a success rate as low as 43% and relatively few complete sapovirus genome sequences are available to improve current typing protocols. OBJECTIVE/STUDY DESIGN: To increase the number of complete sapovirus genomes to better understand the molecular epidemiology of human sapovirus and to improve the success rate of current sapovirus typing methods, we used deep metagenomics shotgun sequencing to obtain the complete genomes of 68 sapovirus samples from four different countries across the Americas (Guatemala, Nicaragua, Peru and the US). RESULTS: VP1 genotyping showed that all sapovirus sequences could be grouped in the four established genogroups (GI (n = 13), GII (n = 30), GIV (n = 23), GV (n = 2)) that infect humans. They include the near-complete genome of a GI.6 virus and a recently reported novel GII.8 virus. Sequences of the complete RNA-dependent RNA polymerase gene could be grouped into three major genetic clusters or polymerase (P) types (GI.P, GII.P and GV.P) with all GIV viruses harboring a GII polymerase. One (GII.P-GII.4) of the new 68 sequences was a recombinant virus with the hotspot between the NS7 and VP1 regions. CONCLUSIONS: Analyses of this expanded database of near-complete sapovirus sequences showed several mismatches in the genotyping primers, suggesting opportunities to revisit and update current sapovirus typing methods. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof urn:issn:1873-5967
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Genotypes en_US
dc.subject Next generation sequencing en_US
dc.subject Sapovirus en_US
dc.subject Viral gastroenteritis en_US
dc.title Genetic diversity of human sapovirus across the Americas en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.jcv.2018.05.003
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.02


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