Universidad Peruana Cayetano Heredia

Immunostimulatory activity of low-molecular-weight hyaluronan on dendritic cells stimulated with Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis

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dc.contributor.author Monasterio, Gustavo
dc.contributor.author Guevara, José
dc.contributor.author Ibarra, Juan Pablo
dc.contributor.author Castillo, Francisca
dc.contributor.author Díaz-Zúñiga, Jaime
dc.contributor.author Alvarez, Carla
dc.contributor.author Cafferata, Emilio A.
dc.contributor.author Vernal, Rolando
dc.date.accessioned 2018-11-30T17:17:13Z
dc.date.available 2018-11-30T17:17:13Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4093
dc.description.abstract OBJECTIVES: Periodontitis is a chronic inflammatory disease characterized by tooth-supporting tissue destruction, which is elicited by the host's immune response triggered against periodonto-pathogen bacteria. During periodontal tissue destruction, extracellular matrix components are metabolized and fragmented. Some extracellular matrix component-derived fragments, such as low-molecular-weight hyaluronan (LMW-HA), have potent immunogenic potential, playing a role as damage-associated molecular patterns (DAMPs) during activation of immune cells. Dendritic cells (DCs) play a central role in the host's immune response displayed during periodontitis; thus, this study aimed to analyze whether LMW-HA has an immunostimulatory activity on DCs when stimulated with periodonto-pathogen bacteria. MATERIALS AND METHODS: LMW-HA-treated and non-treated DCs were stimulated with Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis and the mRNA expression for cytokines tumor necrosis factor-α (TNF-alpha), interleukin-1β (IL-1B), interleukin-6 (IL-6), and interleukin-23 (IL-23A) was quantified by RT-qPCR. In addition, transcription factors interferon regulatory factor 4 (IRF4), interferon regulatory factor 8 (IRF8), neurogenic locus notch homolog protein 2 (NOTCH2), and basic leucine zipper ATF-like transcription factor 3 (BATF3), involved in DC activation, were analyzed. RESULTS: Higher expression levels of TNF-alpha, IL-1B, IL-6, and IL-23A were detected in LMW-HA-treated DCs after bacterial infection, as compared with non-treated DCs. When LMW-HA-treated DCs were infected with A. actinomycetemcomitans, higher levels of IRF4, NOTCH2, and BATF3 were detected compared with non-treated cells; whereas against P. gingivalis infection, increased levels of IRF4 and NOTCH2 were detected. CONCLUSION: LMW-HA plays an immunostimulatory role on the immune response triggered by DCs during infection with A. actinomycetemcomitans or P. gingivalis. CLINICAL RELEVANCE: Detection of extracellular matrix component-derived fragments produced during periodontal tissue destruction, such as LMW-HA, could explain at least partly unsuccessful periodontal treatment and the chronicity of the disease. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartofseries Clinical Oral Investigations
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Aggregatibacter actinomycetemcomitans en_US
dc.subject Cytokines en_US
dc.subject Dendritic cells en_US
dc.subject Hyaluronan en_US
dc.subject LMW-HA en_US
dc.subject Porphyromonas gingivalis en_US
dc.subject Transcription factors en_US
dc.title Immunostimulatory activity of low-molecular-weight hyaluronan on dendritic cells stimulated with Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s00784-018-2641-5
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.14
dc.relation.issn 1436-3771


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