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Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa

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dc.contributor.author Horna, Gertrudis
dc.contributor.author López, María
dc.contributor.author Guerra, Humberto
dc.contributor.author Saénz, Yolanda
dc.contributor.author Ruiz, Joaquim
dc.date.accessioned 2018-11-30T17:17:14Z
dc.date.available 2018-11-30T17:17:14Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4103
dc.description.abstract MexAB-OprM and MexEF-OprN are Pseudomonas aeruginosa efflux pumps involved in the development of antibiotic resistance. Several studies developed with laboratory strains or using a few clinical isolates have reported that the regulation system of MexEF-OprN is involved in the final levels of MexAB-OprM expression. Therefore, this study was aimed to determine the interplay between MexAB-OprM and MexEF-OprN in 90 out of 190 P. aeruginosa clinical isolates with an efflux pump overexpression phenotype. Regarding oprD, 33% (30/90) of isolates displayed relevant modifications (RM) defined as frameshift or premature stop, both related to carbapenem resistance. On the other hand, 33% of the isolates displayed RM in nalC, nalD or mexR, which were significantly associated with multidrug resistance (MDR), non-susceptibility to carbapenems, OprD alterations and strong biofilm production. Meanwhile, the RM in MexS were associated with presence of pigment (p = 0.004). Otherwise, when all the regulators were analysed together, the association between RM in MexAB-OprM regulators and MDR was only significant (p = 0.039) when mexS was the wild type. These data show the modulatory effect of MexEF-OprN on MexAB-OprM in a clinical population of P. aeruginosa. Further studies may contribute to design of novel molecules acting on this interplay to fight against antimicrobial resistance. en_US
dc.language.iso eng
dc.publisher Springer Nature
dc.relation.ispartofseries Scientific Reports
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Strong Biofilm Producers (SBP) en_US
dc.subject Relevant Modifications en_US
dc.subject oprD Gene en_US
dc.subject Final Expression Levels en_US
dc.subject mexS Gene en_US
dc.title Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1038/s41598-018-34694-z
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.09
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 2045-2322


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