Universidad Peruana Cayetano Heredia
Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa
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| dc.contributor.author | Horna, Gertrudis | |
| dc.contributor.author | López, María | |
| dc.contributor.author | Guerra, Humberto | |
| dc.contributor.author | Saénz, Yolanda | |
| dc.contributor.author | Ruiz, Joaquim | |
| dc.date.accessioned | 2018-11-30T17:17:14Z | |
| dc.date.available | 2018-11-30T17:17:14Z | |
| dc.date.issued | 2018 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12866/4103 | |
| dc.description.abstract | MexAB-OprM and MexEF-OprN are Pseudomonas aeruginosa efflux pumps involved in the development of antibiotic resistance. Several studies developed with laboratory strains or using a few clinical isolates have reported that the regulation system of MexEF-OprN is involved in the final levels of MexAB-OprM expression. Therefore, this study was aimed to determine the interplay between MexAB-OprM and MexEF-OprN in 90 out of 190 P. aeruginosa clinical isolates with an efflux pump overexpression phenotype. Regarding oprD, 33% (30/90) of isolates displayed relevant modifications (RM) defined as frameshift or premature stop, both related to carbapenem resistance. On the other hand, 33% of the isolates displayed RM in nalC, nalD or mexR, which were significantly associated with multidrug resistance (MDR), non-susceptibility to carbapenems, OprD alterations and strong biofilm production. Meanwhile, the RM in MexS were associated with presence of pigment (p = 0.004). Otherwise, when all the regulators were analysed together, the association between RM in MexAB-OprM regulators and MDR was only significant (p = 0.039) when mexS was the wild type. These data show the modulatory effect of MexEF-OprN on MexAB-OprM in a clinical population of P. aeruginosa. Further studies may contribute to design of novel molecules acting on this interplay to fight against antimicrobial resistance. | en_US |
| dc.language.iso | eng | |
| dc.publisher | Springer Nature | |
| dc.relation.ispartofseries | Scientific Reports | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.subject | Strong Biofilm Producers (SBP) | en_US |
| dc.subject | Relevant Modifications | en_US |
| dc.subject | oprD Gene | en_US |
| dc.subject | Final Expression Levels | en_US |
| dc.subject | mexS Gene | en_US |
| dc.title | Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa | en_US |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | https://doi.org/10.1038/s41598-018-34694-z | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.01.09 | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.06.01 | |
| dc.relation.issn | 2045-2322 |
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