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Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum

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dc.contributor.author Muñoz-Atienza, E.
dc.contributor.author Távara, C.
dc.contributor.author Díaz-Rosales, P.
dc.contributor.author Llanco, L.
dc.contributor.author Serrano-Martínez, E.
dc.contributor.author Tafalla, C.
dc.date.accessioned 2018-11-30T17:17:15Z
dc.date.available 2018-11-30T17:17:15Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4111
dc.description.abstract Flavobacterium psychrophilum is the etiological agent of bacterial cold water disease (BCWD), also referred to as rainbow trout fry syndrome (RTFS), a disease with great economic impact in salmonid aquaculture. Despite this, to date, not many studies have analyzed in depth how the immune system is regulated during the course of the disease. In the current study, we have studied the transcription of several immune genes related to T and B cell activity in the skin of rainbow trout (Oncorhynchus mykiss) naturally infected with F. psychrophilum in a farm located in Lake Titicaca (Peru). The levels of expression of these genes were tested and compared to those obtained in asymptomatic and apparently healthy rainbow trout. In the case of symptomatic fish, skin samples containing characteristic ulcerative lesions were taken, as well as skin samples with no lesions. Our results pointed to a significant local up-regulation of IgD, CD4, CD8, perforin and IFNγ within the ulcerative lesions. On the other hand, no differences between the levels of expression of these genes were visible in the spleen. To confirm these results, the distribution of IgD+ and CD3+ cells was studied through immunohistochemical techniques in the ulcerative lesions. Our results demonstrate a strong local response to F. psychrophilum in rainbow trout in which IgD and T cells seem to play a major role. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof urn:issn:1095-9947
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject adult en_US
dc.subject animal en_US
dc.subject animal cell en_US
dc.subject animal experiment en_US
dc.subject animal tissue en_US
dc.subject Animals en_US
dc.subject Article en_US
dc.subject B lymphocyte en_US
dc.subject Bacterial cold water disease (BCWD) en_US
dc.subject bacterial RNA en_US
dc.subject CD3+ T lymphocyte en_US
dc.subject CD4 antigen en_US
dc.subject CD8 antigen en_US
dc.subject comparative study en_US
dc.subject complementary DNA en_US
dc.subject controlled study en_US
dc.subject cytokine en_US
dc.subject Cytokines en_US
dc.subject DNA 16S en_US
dc.subject elongation factor 1alpha en_US
dc.subject fish disease en_US
dc.subject Fish Diseases en_US
dc.subject fish genetics en_US
dc.subject Flavobacteriaceae infection en_US
dc.subject Flavobacteriaceae Infections en_US
dc.subject Flavobacterium en_US
dc.subject Flavobacterium psychrophilum en_US
dc.subject gamma interferon en_US
dc.subject gene expression regulation en_US
dc.subject Gene Expression Regulation en_US
dc.subject genetics en_US
dc.subject glyceraldehyde 3 phosphate dehydrogenase en_US
dc.subject immune response en_US
dc.subject immunoglobulin en_US
dc.subject immunoglobulin D en_US
dc.subject Immunoglobulin D (IgD) en_US
dc.subject immunoglobulin M en_US
dc.subject Immunoglobulins en_US
dc.subject immunohistochemistry en_US
dc.subject immunology en_US
dc.subject immunoregulation en_US
dc.subject Inflammatory response en_US
dc.subject interleukin 13 en_US
dc.subject interleukin 4 en_US
dc.subject messenger RNA en_US
dc.subject metabolism en_US
dc.subject microbiology en_US
dc.subject mRNA expression level en_US
dc.subject nonhuman en_US
dc.subject Oncorhynchus mykiss en_US
dc.subject perforin en_US
dc.subject priority journal en_US
dc.subject Rainbow trout en_US
dc.subject rainbow trout fry syndrome en_US
dc.subject RNA 16S en_US
dc.subject RNA, Bacterial en_US
dc.subject RNA, Ribosomal, 16S en_US
dc.subject Skin en_US
dc.subject skin ulcer en_US
dc.subject spleen en_US
dc.subject Spleen en_US
dc.subject T cells en_US
dc.subject T lymphocyte en_US
dc.subject transcription factor FOXP3 en_US
dc.subject transcription regulation en_US
dc.subject upregulation en_US
dc.subject veterinary medicine en_US
dc.title Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.fsi.2018.11.027
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.12
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.03


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