DSpace Repository

Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

Show simple item record

dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Castillo, Yesenia
dc.contributor.author Gonzales, Isidro
dc.contributor.author Bustos Palomino, Javier Arturo
dc.contributor.author Saavedra Pastor, Herbert
dc.contributor.author Jacob, Louis
dc.contributor.author Del Brutto, Oscar H.
dc.contributor.author Wilkins, Patricia P.
dc.contributor.author Gonzalez, Armando E.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Cysticercosis Working Group in Peru
dc.date.accessioned 2018-11-30T17:17:15Z
dc.date.available 2018-11-30T17:17:15Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4113
dc.description.abstract OBJECTIVE: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa® and Ridascreen® , for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). METHODS: Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa® and Ridascreen® . All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. RESULTS: Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). CONCLUSIONS: The performance of Novalisa® and Ridascreen® was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis. en_US
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof urn:issn:1365-3156
dc.relation.ispartofseries Tropical Medicine and International Health
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Peru en_US
dc.subject Neurocysticercosis en_US
dc.subject Taenia solium en_US
dc.subject neurocysticercosis en_US
dc.subject Animals en_US
dc.subject Antigens, Helminth en_US
dc.subject Enzyme-Linked Immunosorbent Assay en_US
dc.subject Sensitivity and Specificity en_US
dc.subject antibody en_US
dc.subject cysticercosis en_US
dc.subject ELISA en_US
dc.subject anticorps en_US
dc.subject cysticercose en_US
dc.subject Immunologic Tests en_US
dc.subject Immunomagnetic Separation en_US
dc.subject neurocysticercose en_US
dc.subject Pérou en_US
dc.title Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1111/tmi.13010
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


My Account