Universidad Peruana Cayetano Heredia
MODS-Wayne, a colorimetric adaptation of the Microscopic-Observation Drug Susceptibility (MODS) assay for detection of mycobacterium tuberculosis pyrazinamide resistance from sputum samples
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| dc.contributor.author | Alcántara, Roberto | |
| dc.contributor.author | Fuentes, Patricia | |
| dc.contributor.author | Antiparra, Ricardo | |
| dc.contributor.author | Santos, Marco | |
| dc.contributor.author | Gilman, Robert Hugh | |
| dc.contributor.author | Kirwan, Daniela E. | |
| dc.contributor.author | Zimic-Peralta, Mirko Juan | |
| dc.contributor.author | Sheen Cortavarria, Patricia | |
| dc.date.accessioned | 2018-11-30T17:17:15Z | |
| dc.date.available | 2018-11-30T17:17:15Z | |
| dc.date.issued | 2018 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12866/4120 | |
| dc.description.abstract | Although pyrazinamide (PZA) is a key component of first and second line tuberculosis treatment regimens, there is no gold standard to determine PZA resistance. Approximately 50% of multidrug-resistant tuberculosis (MDR-TB) and over 90% of extensively drug resistant tuberculosis (XDR-TB) strains are also PZA resistant. pncA sequencing is the endorsed test to evaluate PZA susceptibility. However, molecular methods have limitations for their wide application.In this study we standardized and evaluated a new method to determine PZA resistance: MODS-Wayne. MODS-Wayne is based on the detection of pyrazinoic acid, the hydrolysis product of PZA, directly in the supernatant of sputum cultures by detecting a color change following addition of 10% ferrous ammonium sulfate. Using a PZA concentration of 800 µg/mL, sensitivity and specificity were evaluated at three time different periods of incubation (Reading 1, reading 2, reading 3)) using a composite standard (MGIT-PZA, pncA sequencing, and the classic Wayne test).MODS-Wayne was able to detect PZA resistance with a sensitivity and specificity of 92.7% and 99.3% respectively at Reading 3. MODS-Wayne had an agreement of 93.8% and a kappa index of 0.79 compared to the classic Wayne test, agreement of 95.3% and kappa index of 0.86 compared to MGIT-PZA, and agreement of 96.9% and kappa index of 0.90 compared to pncA sequencing. In conclusion, MODS-Wayne is a simple, fast, accurate, and inexpensive approach to detect PZA resistance, making this an attractive assay especially for low resource countries, where TB is a major public health problem. | en_US |
| dc.language.iso | eng | |
| dc.publisher | American Society for Microbiology | |
| dc.relation.ispartofseries | Journal of Clinical Microbiology | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.subject | ammonium sulfate | en_US |
| dc.subject | antibiotic resistance | en_US |
| dc.subject | antibiotic sensitivity | en_US |
| dc.subject | Article | en_US |
| dc.subject | colorimetry | en_US |
| dc.subject | concentration (parameter) | en_US |
| dc.subject | controlled study | en_US |
| dc.subject | drug hydrolysis | en_US |
| dc.subject | incubation time | en_US |
| dc.subject | intermethod comparison | en_US |
| dc.subject | isoniazid | en_US |
| dc.subject | microscopy | en_US |
| dc.subject | MODS | en_US |
| dc.subject | Mycobacterium tuberculosis | en_US |
| dc.subject | nonhuman | en_US |
| dc.subject | priority journal | en_US |
| dc.subject | pyrazinamide | en_US |
| dc.subject | Pyrazinamide | en_US |
| dc.subject | pyrazinoic acid | en_US |
| dc.subject | Pyrazinoic acid | en_US |
| dc.subject | rifampicin | en_US |
| dc.subject | sensitivity and specificity | en_US |
| dc.subject | sequence analysis | en_US |
| dc.subject | Sputum | en_US |
| dc.subject | sputum analysis | en_US |
| dc.subject | sputum culture | en_US |
| dc.subject | standard | en_US |
| dc.subject | supernatant | en_US |
| dc.subject | Tuberculosis | en_US |
| dc.title | MODS-Wayne, a colorimetric adaptation of the Microscopic-Observation Drug Susceptibility (MODS) assay for detection of mycobacterium tuberculosis pyrazinamide resistance from sputum samples | en_US |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | https://doi.org/10.1128/JCM.01162-18 | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.06.01 | |
| dc.relation.issn | 1098-660X |
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