Molecular Detection of Bartonella bacilliformis in Lutzomyia maranonensis in Cajamarca, Peru: A New Potential Vector of Carrion's Disease in Peru?

Ulloa, Gabriela M.; Gomes, Cláudia; Del Valle Mendoza, Juana; Ruiz, Joaquim; Vásquez-Achaya, Fernando; Del Valle, Luis J.; Pons, Maria J.

dc.contributor.author	Ulloa, Gabriela M.
dc.contributor.author	Vásquez-Achaya, Fernando
dc.contributor.author	Gomes, Cláudia
dc.contributor.author	Del Valle, Luis J.
dc.contributor.author	Ruiz, Joaquim
dc.contributor.author	Pons, Maria J.
dc.contributor.author	Del Valle Mendoza, Juana
dc.date.accessioned	2018-11-30T17:17:16Z
dc.date.available	2018-11-30T17:17:16Z
dc.date.issued	2018
dc.identifier.uri	https://hdl.handle.net/20.500.12866/4123
dc.description.abstract	Carrion's disease is a neglected, vector-borne illness that affects Colombia, Ecuador, and especially Peru. The phlebotomine sand flies Lutzomyia verrucarum and Lutzomyia peruensis are the main illness vectors described, although other species may be implicated in endemic areas such as some northern Peruvian regions, in which Carrion's disease vector has not been established. The aim of this study was to evaluate the presence of Bartonella bacilliformis DNA in Lutzomyia maranonensis from Cajamarca, northern Peru. This sand fly has not been defined as a vector yet. Centers for Disease Control and Prevention light traps were used to collect adult phlebotomine sand flies from 2007 to 2008 in the Cajamarca department. Female specimens were identified using morphological keys and were grouped into pools of five sand flies, taking into account district and sampling site (intradomicile or peridomicile). DNA was extracted, and then conventional and real-time polymerase chain reaction (RT-PCR) were performed to detect B. bacilliformis and subsequently confirmed by sequencing. A total of 383 specimens of L. maranonensis species were analyzed. Two of 76 pools were positive for B. bacilliformis by sequencing; all positives pools were from Querocotillo district. In addition, Mesorhizobium spp. were identified in two pools of sand flies, which is an α-proteobacteria phylogenetically very close to B. bacilliformis. This study presents molecular evidence that suggests L. maranonensis is naturally infected by B. bacilliformis in the Cajamarca department. Further research should determine if L. maranonensis is a vector and could transmit B. bacilliformis.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Molecular Detection	en_US
dc.subject	Bartonella bacilliformis	en_US
dc.subject	Lutzomyia maranonensis	en_US
dc.subject	Cajamarca	en_US
dc.subject	Peru	en_US
dc.subject	Carrion’s Disease	en_US
dc.title	Molecular Detection of Bartonella bacilliformis in Lutzomyia maranonensis in Cajamarca, Peru: A New Potential Vector of Carrion's Disease in Peru?	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.18-0520
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645