Molecular Detection of Taeniid Eggs in Beetles Collected in an Area Endemic for Taenia solium

Gomez-Puerta, Luis A.; García Lescano, Héctor Hugo; Vargas-Calla, Ana; Cysticercosis Working Group in Peru; Pajuelo Travezaño, Monica Jhenny; Gonzalez Zariquiey, Armando Emiliano

dc.contributor.author	Vargas-Calla, Ana
dc.contributor.author	Gomez-Puerta, Luis A.
dc.contributor.author	Pajuelo Travezaño, Monica Jhenny
dc.contributor.author	García Lescano, Héctor Hugo
dc.contributor.author	Gonzalez Zariquiey, Armando Emiliano
dc.contributor.author	Cysticercosis Working Group in Peru
dc.date.accessioned	2018-11-30T17:17:16Z
dc.date.available	2018-11-30T17:17:16Z
dc.date.issued	2018
dc.identifier.uri	https://hdl.handle.net/20.500.12866/4124
dc.description.abstract	The aim of this study was to demonstrate the presence of Taenia solium eggs in beetles collected from sources within the natural environment through molecular techniques. Fifty-four pools of beetles were collected in three villages in Piura, Peru. DNA was extracted using the FastDNA spin kit for soil. Molecular identification of Taenia species was then performed through partial amplification of the mitochondrial cytochrome C oxidase subunit I gene. Finally, positive samples were sequenced to determine the tapeworm species. Seven positive samples were obtained through polymerase chain reaction amplification. Sequencing confirmed that two samples were from T. solium and three samples were from Taenia hydatigena. The other two samples could not be specifically identified. Our findings demonstrate that dung beetles ingest T. solium and T. hydatigena eggs under natural conditions and suggest that beetles may play a role in the dynamics of transmission of these cestodes.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Molecular Detection	en_US
dc.subject	Taeniid Eggs	en_US
dc.subject	Beetles	en_US
dc.subject	Taenia solium	en_US
dc.title	Molecular Detection of Taeniid Eggs in Beetles Collected in an Area Endemic for Taenia solium	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.18-0355
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645