Universidad Peruana Cayetano Heredia

Molecular Detection of Taeniid Eggs in Beetles Collected in an Area Endemic for Taenia solium

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dc.contributor.author Vargas-Calla, Ana
dc.contributor.author Gomez-Puerta, Luis A.
dc.contributor.author Pajuelo Travezaño, Monica Jhenny
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Gonzalez Zariquiey, Armando Emiliano
dc.contributor.author Cysticercosis Working Group in Peru
dc.date.accessioned 2018-11-30T17:17:16Z
dc.date.available 2018-11-30T17:17:16Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4124
dc.description.abstract The aim of this study was to demonstrate the presence of Taenia solium eggs in beetles collected from sources within the natural environment through molecular techniques. Fifty-four pools of beetles were collected in three villages in Piura, Peru. DNA was extracted using the FastDNA spin kit for soil. Molecular identification of Taenia species was then performed through partial amplification of the mitochondrial cytochrome C oxidase subunit I gene. Finally, positive samples were sequenced to determine the tapeworm species. Seven positive samples were obtained through polymerase chain reaction amplification. Sequencing confirmed that two samples were from T. solium and three samples were from Taenia hydatigena. The other two samples could not be specifically identified. Our findings demonstrate that dung beetles ingest T. solium and T. hydatigena eggs under natural conditions and suggest that beetles may play a role in the dynamics of transmission of these cestodes. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Molecular Detection en_US
dc.subject Taeniid Eggs en_US
dc.subject Beetles en_US
dc.subject Taenia solium en_US
dc.title Molecular Detection of Taeniid Eggs in Beetles Collected in an Area Endemic for Taenia solium en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.18-0355
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1476-1645


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