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Profiling of Hura crepitans L. latex by ultra-high-performance liquid chromatography/atmospheric pressure chemical ionisation linear ion trap Orbitrap mass spectrometry

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dc.contributor.author Trinel, Manon
dc.contributor.author Jullian, Valérie
dc.contributor.author Le Lamer, Anne-Cécile
dc.contributor.author Mhamdi, Icram
dc.contributor.author Mejia, Kember
dc.contributor.author Castillo, Denis
dc.contributor.author Cabanillas, Billy Joel
dc.contributor.author Fabre, Nicolas
dc.date.accessioned 2018-11-30T22:50:36Z
dc.date.available 2018-11-30T22:50:36Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4159
dc.description.abstract INTRODUCTION: The phytochemistry of the latex of Hura crepitans L. (Euphorbiaceae), a widespread tree in the Amazonian forest having many uses, is little known. Only huratoxin, a daphnane diterpene orthoester, has been described despite the high pharmacological potential of this kind of compounds. Glucosphingolipids (cerebrosides) are also known to be distributed in Euphorbiaceae latexes. OBJECTIVE: To tentatively identify daphnanes diterpenes and cerebrosides in the latex of H. crepitans. METHODS: An ethanolic extract of the lyophilised latex of H. crepitans was analysed by ultra-high-performance liquid chromatography (UHPLC) coupled with positive and negative atmospheric pressure chemical ionisation high-resolution mass spectrometry (APCI-HRMS) method using a quadrupole/linear ion trap/Orbitrap (LTQ-Orbitrap). Tandem mass spectrometry (MS/MS) spectra were recorded by two different fragmentation modes: collision induced dissociation (CID) and higher-energy collisional dissociation (HCD). RESULTS: The analysis of CID- and HCD-MS/MS spectra allowed to propose fragmentation patterns for daphnane esters and cerebrosides and highlight diagnostic ions in positive and negative ion modes. A total of 34 compounds including 24 daphnane esters and 10 cerebrosides have been tentatively annotated. Among them, 17 daphnane diterpenes bearing one or two acyl chains are new compounds and the cerebrosides are described in the genus Hura for the first time. CONCLUSION: This study revealed the chemical constituents of the latex of H. crepitans and particularly its richness and chemical diversity in daphnane diterpenes, more frequently encountered in the species of Thymelaeaceae. en_US
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof urn:issn:1099-1565
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject cerebrosides en_US
dc.subject Chromatography, Liquid en_US
dc.subject daphnane diterpenes en_US
dc.subject Euphorbiaceae en_US
dc.subject fragmentation patterns en_US
dc.subject Hura crepitans en_US
dc.subject Latex en_US
dc.subject Mass Spectrometry en_US
dc.subject Molecular Structure en_US
dc.subject Plant Extracts en_US
dc.subject Toxins, Biological en_US
dc.subject UHPLC-MS/MS en_US
dc.title Profiling of Hura crepitans L. latex by ultra-high-performance liquid chromatography/atmospheric pressure chemical ionisation linear ion trap Orbitrap mass spectrometry en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1002/pca.2776
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.10
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.03
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.04.07
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.29

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