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Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry

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dc.contributor.author Steinberg, Hannah E.
dc.contributor.author Russo, Paul
dc.contributor.author Angulo, Noelia
dc.contributor.author Ynocente, Raúl
dc.contributor.author Montoya, Cristina
dc.contributor.author Diestra, Andrea
dc.contributor.author Ferradas, Cusi
dc.contributor.author Schiaffino, Francesca
dc.contributor.author Florentini, Edgar
dc.contributor.author Jimenez, Juan
dc.contributor.author Calderón, Maritza
dc.contributor.author Carruthers, Vern B.
dc.contributor.author Gilman, Robert H.
dc.contributor.author Liotta, Lance
dc.contributor.author Luchini, Alessandra
dc.date.accessioned 2018-11-30T23:41:30Z
dc.date.available 2018-11-30T23:41:30Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4200
dc.description.abstract Diagnosis of clinical toxoplasmosis remains a challenge, thus limiting the availability of human clinical samples. Though murine models are an approximation of human response, their definitive infection status and tissue availability make them critical to the diagnostic development process. Hydrogel mesh nanoparticles were used to concentrate antigen to detectable levels for mass spectrometry. Seven Toxoplasma gondii isolates were used to develop a panel of potential peptide sequences for detection by parallel reaction monitoring (PRM) mass spectrometry. Nanoparticles were incubated with decreasing concentrations of tachyzoite lysate to explore the limits of detection of PRM. Mice whose toxoplasmosis infection status was confirmed by quantitative real-time PCR had urine tested by PRM after hydrogel mesh concentration for known T. gondii peptides. Peptides from GRA1, GRA12, ROP4, ROP5, SAG1, and SAG2A proteins were detected by PRM after nanoparticle concentration of urine, confirming detection of T. gondii antigen in the urine of an infected mouse. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof urn:issn:1549-9642
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Multiple reactions monitoring en_US
dc.subject Nanoparticles en_US
dc.subject Toxoplasma gondii en_US
dc.title Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.nano.2017.11.020
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE


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