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An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle

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dc.contributor.author Campero, Lucia M.
dc.contributor.author Moreno-Gonzalo, Javier
dc.contributor.author Venturini, Maria C.
dc.contributor.author More, Gaston
dc.contributor.author Dellarupe, Andrea
dc.contributor.author Rambeaud, Magdalena
dc.contributor.author Echaide, Ignacio E.
dc.contributor.author Valentini, Beatriz
dc.contributor.author Campero, Carlos M.
dc.contributor.author Moore, Dadin P.
dc.contributor.author Cano, Dora B.
dc.contributor.author Fort, Marcelo
dc.contributor.author Mota, Rinaldo A.
dc.contributor.author Serrano Martinez, Marcos Enrique
dc.contributor.author Cruz-Vazquez, Carlos
dc.contributor.author Ortega-Mora, Luis M.
dc.contributor.author Alvarez-Garcia, Gema
dc.date.accessioned 2018-11-30T23:41:31Z
dc.date.available 2018-11-30T23:41:31Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4215
dc.description.abstract We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1-7) and three enzyme-linked immunosorbent assays (ELISAs 1-3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the “Pre-test information,” which uses previous epidemiological and serological data; (2) the “Majority of tests,” which classifies a serum as positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartofseries Tropical Animal Health and Production
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Bovine neosporosis en_US
dc.subject Ibero-America en_US
dc.subject Serological assay trial en_US
dc.title An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s11250-017-1401-x
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#4.03.00
dc.relation.issn 1573-7438


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