dc.contributor.author |
Campero, Lucia M. |
|
dc.contributor.author |
Moreno-Gonzalo, Javier |
|
dc.contributor.author |
Venturini, Maria C. |
|
dc.contributor.author |
More, Gaston |
|
dc.contributor.author |
Dellarupe, Andrea |
|
dc.contributor.author |
Rambeaud, Magdalena |
|
dc.contributor.author |
Echaide, Ignacio E. |
|
dc.contributor.author |
Valentini, Beatriz |
|
dc.contributor.author |
Campero, Carlos M. |
|
dc.contributor.author |
Moore, Dadin P. |
|
dc.contributor.author |
Cano, Dora B. |
|
dc.contributor.author |
Fort, Marcelo |
|
dc.contributor.author |
Mota, Rinaldo A. |
|
dc.contributor.author |
Serrano Martinez, Marcos Enrique |
|
dc.contributor.author |
Cruz-Vazquez, Carlos |
|
dc.contributor.author |
Ortega-Mora, Luis M. |
|
dc.contributor.author |
Alvarez-Garcia, Gema |
|
dc.date.accessioned |
2018-11-30T23:41:31Z |
|
dc.date.available |
2018-11-30T23:41:31Z |
|
dc.date.issued |
2018 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/4215 |
|
dc.description.abstract |
We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1-7) and three enzyme-linked immunosorbent assays (ELISAs 1-3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the “Pre-test information,” which uses previous epidemiological and serological data; (2) the “Majority of tests,” which classifies a serum as positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
Springer |
|
dc.relation.ispartofseries |
Tropical Animal Health and Production |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
Bovine neosporosis |
en_US |
dc.subject |
Ibero-America |
en_US |
dc.subject |
Serological assay trial |
en_US |
dc.title |
An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.1007/s11250-017-1401-x |
|
dc.subject.ocde |
https://purl.org/pe-repo/ocde/ford#4.03.00 |
|
dc.relation.issn |
1573-7438 |
|