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Anti-helicobacter pylori properties of the ant-venom peptide bicarinalin

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dc.contributor.author Guzman, J.
dc.contributor.author Téné, N.
dc.contributor.author Touchard, A.
dc.contributor.author Castillo, D.
dc.contributor.author Belkhelfa, H.
dc.contributor.author Haddioui-Hbabi, L.
dc.contributor.author Treilhou, M.
dc.contributor.author Sauvain, M.
dc.date.accessioned 2018-11-30T23:41:31Z
dc.date.available 2018-11-30T23:41:31Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4218
dc.description.abstract The venom peptide bicarinalin, previously isolated from the ant Tetramorium bicarinatum, is an antimicrobial agent with a broad spectrum of activity. In this study, we investigate the potential of bicarinalin as a novel agent against Helicobacter pylori, which causes several gastric diseases. First, the effects of synthetic bicarinalin have been tested against Helicobacter pylori: one ATCC strain, and forty-four isolated from stomach ulcer biopsies of Peruvian patients. Then the cytoxicity of bicarinalin on human gastric cells and murine peritoneal macrophages was measured using XTT and MTT assays, respectively. Finally, the preventive effect of bicarinalin was evaluated by scanning electron microscopy using an adherence assay of H. pylori on human gastric cells treated with bicarinalin. This peptide has a potent antibacterial activity at the same magnitude as four antibiotics currently used in therapies against H. pylori. Bicarinalin also inhibited adherence of H. pylori to gastric cells with an IC50 of 0.12 μg·mL–1 and had low toxicity for human cells. Scanning electron microscopy confirmed that bicarinalin can significantly decrease the density of H. pylori on gastric cells. We conclude that Bicarinalin is a promising compound for the development of a novel and effective anti-H. pylori agent for both curative and preventive use. en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartof urn:issn:2072-6651
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject levofloxacin en_US
dc.subject human en_US
dc.subject Helicobacter pylori en_US
dc.subject Article en_US
dc.subject nonhuman en_US
dc.subject bacterial strain en_US
dc.subject unclassified drug en_US
dc.subject amoxicillin en_US
dc.subject animal cell en_US
dc.subject antibacterial activity en_US
dc.subject Antimicrobial peptide en_US
dc.subject Bacterial adhesion en_US
dc.subject bacterial growth en_US
dc.subject bicarinalin en_US
dc.subject Bicarinalin en_US
dc.subject CC50 en_US
dc.subject clarithromycin en_US
dc.subject cytotoxicity en_US
dc.subject drug synthesis en_US
dc.subject Gastric cells en_US
dc.subject human cell en_US
dc.subject macrophage en_US
dc.subject metronidazole en_US
dc.subject minimum inhibitory concentration en_US
dc.subject mouse en_US
dc.subject MTT assay en_US
dc.subject scanning electron microscopy en_US
dc.subject selectivity index en_US
dc.subject SEM en_US
dc.subject venom en_US
dc.subject XTT assay en_US
dc.subject antimicrobial peptide en_US
dc.subject bacterial adhesion en_US
dc.subject gastric cells en_US
dc.title Anti-helicobacter pylori properties of the ant-venom peptide bicarinalin en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/toxins10010021
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.07

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