Universidad Peruana Cayetano Heredia

Low-cost diagnostic test for susceptible and drug-resistant tuberculosis in rural Malawi

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dc.contributor.author Zhang, Annie
dc.contributor.author Jumbe, Enoch
dc.contributor.author Krysiak, Robert
dc.contributor.author Sidiki, Sabeen
dc.contributor.author Kelley, Holden V.
dc.contributor.author Chemey, Elly K.
dc.contributor.author Kamba, Chancy
dc.contributor.author Mwapasa, Victor
dc.contributor.author Garcia, Juan I.
dc.contributor.author Norris, Alison
dc.contributor.author Pan, Xueliang J.
dc.contributor.author Evans, Carlton Anthony William
dc.contributor.author Wang, Shu-Hua
dc.contributor.author Kwiek, Jesse J.
dc.contributor.author Torrelles, Jordi B.
dc.date.accessioned 2018-12-01T00:04:14Z
dc.date.available 2018-12-01T00:04:14Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/4247
dc.description.abstract Background: Rural settings where molecular tuberculosis diagnostics are not currently available need easy-to-use tests that do not require additional processing or equipment. While acid-fast bacilli (AFB) smear is the most common and often only tuberculosis diagnosis test performed in rural settings, it is labour intensive, has less-than-ideal sensitivity, and cannot assess tuberculosis drug susceptibility patterns. Objective: The objective of this study was to determine the feasibility of a multidrugresistant (MDR) or extensively drug-resistant (XDR)-tuberculosis coloured agar-based culture test (tuberculosis CX-test), which can detect Mycobacterium tuberculosis growth and evaluate for drug susceptibility to isoniazid, rifampicin and a fluoroquinolone (i. e. ciprofloxacin) in approximately 14 days. Method: In this study, 101 participants were enrolled who presented to a rural health clinic in central Malawi. They were suspected of having active pulmonary tuberculosis. Participants provided demographic and clinical data and submitted sputum samples for tuberculosis testing using the AFB smear and tuberculosis CX-test. Results: The results showed a high level of concordance between the AFB smear (12 positive) and tuberculosis CX-test (13 positive); only one sample presented discordant results, with the molecular GeneXpert MTB/RIF (R) test confirming the tuberculosis CX-test results. The average time to a positive tuberculosis CX-test was 10 days. Of the positive samples, the tuberculosis CXtest detected no cases of drug resistance, which was later confirmed by the GeneXpert MTB/RIF (R). Conclusion: These findings demonstrate that the tuberculosis CX-test could be a reliable low-cost diagnostic method for active pulmonary tuberculosis in high tuberculosis burden rural areas. en_US
dc.language.iso eng
dc.publisher African Online Scientific Information Systems
dc.relation.ispartofseries African Journal of Laboratory Medicine
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Tuberculosis en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject diagnosis en_US
dc.subject drug resistance en_US
dc.title Low-cost diagnostic test for susceptible and drug-resistant tuberculosis in rural Malawi en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4102/ajlm.v7i1.690
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.03
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#2.06.02
dc.relation.issn 2225-2010


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