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Anti-Fas2 IgM antibodies in Fasciola hepatica infected patients with positive IgG serology

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dc.contributor.author Kazantseva, Liliya
dc.contributor.author García Lázaro, María Del Pilar
dc.contributor.author Herrera-Velit, Patricia
dc.contributor.author Espinoza, Jose R.
dc.date.accessioned 2019-01-25T15:02:17Z
dc.date.available 2019-01-25T15:02:17Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/20.500.12866/4600
dc.description.abstract Background: Fascioliasis is an infectious disease caused by parasites Fasciola hepatica and F. gigantica. Humans are infected by the consumption of vegetables and water contaminated with the infective form of the parasite. Materials and Methods: In this study, an IgM-ELISA with the cysteine proteinase Fas2 antigen was evaluated with sera from 76 patients infected with F. hepatica, 24 patients with other parasite infections and 84 healthy volunteers. Results: IgM-ELISA resulted in 43% positives in F. hepatica patients with positive serology to Fas2-ELISA, but no positives resulted from testing healthy volunteers and individuals infected with other parasites. The IgM-ELISA diagnostic parameters showed a sensitivity of 43.4% (95% CI 0.321-0.553), specificity of 100% (95% CI 0.957-1), and no cross-reactivity with other parasitic infection. Interference by rheumatoid factor in the IgM immunoassay was controlled by treating sera with rheumatoid factor absorbent before testing. Conclusions: Fas2 antigen is detected by circulating IgM in patients infected with F. hepatica and IgM-ELISA using Fas2 appears as a specific immunoassay to detect the acute phase of the acute phase of F. hepatica infection in humans. en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartof urn:issn:1878-3503
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Cysteine proteinases en_US
dc.subject ELISA en_US
dc.subject Fascioliasis en_US
dc.subject IgM en_US
dc.subject Immunodiagnosis en_US
dc.subject Rheumatoid factors en_US
dc.subject Animals en_US
dc.subject Antibodies, Anti-Idiotypic/blood en_US
dc.subject Antibodies, Helminth/blood en_US
dc.subject Antigens, Helminth/blood en_US
dc.subject Area Under Curve en_US
dc.subject Cysteine Endopeptidases/blood en_US
dc.subject Enzyme-Linked Immunosorbent Assay en_US
dc.subject Fasciola hepatica/enzymology/immunology en_US
dc.subject Fascioliasis/blood/immunology en_US
dc.subject Humans en_US
dc.subject Immunoglobulin G/blood en_US
dc.subject Immunoglobulin M/immunology en_US
dc.subject Immunologic Factors/pharmacology en_US
dc.subject Peru en_US
dc.subject Predictive Value of Tests en_US
dc.subject Rheumatoid Factor/pharmacology en_US
dc.subject ROC Curve en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Seroepidemiologic Studies en_US
dc.title Anti-Fas2 IgM antibodies in Fasciola hepatica infected patients with positive IgG serology en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1093/trstmh/trx024
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE

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