Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru

Xi, Chuanwu; Baker, Laurence H.; Novoa Reyes, Italo; Sexton, Rachael; Bussalleu Rivera, Alejandro Joaquin; Valdivieso, Manuel; Boehnke, Kevin F.; Crowley, John J.; Osorio, Soledad; Thompson, Kathryn C.

dc.contributor.author	Boehnke, Kevin F.
dc.contributor.author	Valdivieso, Manuel
dc.contributor.author	Bussalleu Rivera, Alejandro Joaquin
dc.contributor.author	Sexton, Rachael
dc.contributor.author	Thompson, Kathryn C.
dc.contributor.author	Osorio, Soledad
dc.contributor.author	Novoa Reyes, Italo
dc.contributor.author	Crowley, John J.
dc.contributor.author	Baker, Laurence H.
dc.contributor.author	Xi, Chuanwu
dc.date.accessioned	2019-01-25T15:02:17Z
dc.date.available	2019-01-25T15:02:17Z
dc.date.issued	2017
dc.identifier.uri	https://hdl.handle.net/20.500.12866/4601
dc.description.abstract	OBJECTIVES: Gastric carcinoma is the most common cancer and cause of cancer mortality in Peru. Helicobacter pylori, a bacterium that colonizes the human stomach, is a Group 1 carcinogen due to its causal relationship to gastric carcinoma. While eradication of H. pylori can help prevent gastric cancer, characterizing regional antibiotic resistance patterns is necessary to determine targeted treatment for each region. Thus, we examined primary antibiotic resistance in clinical isolates of H. pylori in Lima, Peru. MATERIALS AND METHODS: H. pylori strains were isolated from gastric biopsies of patients with histologically proven H. pylori infection. Primary antibiotic resistance among isolates was examined using E-test strips. Isolates were examined for the presence of the cagA pathogenicity island and the vacA m1/m2 alleles via polymerase chain reaction. RESULTS: Seventy-six isolates were recovered from gastric biopsies. Clinical isolates showed evidence of antibiotic resistance to 1 (27.6%, n=21/76), 2 (28.9%, n=22/76), or >/=3 antibiotics (40.8%). Of 76 isolates, eight (10.5%) were resistant to amoxicillin and clarithromycin, which are part of the standard triple therapy for H. pylori infection. No trends were seen between the presence of cagA, vacA m1, or vacA m2 and antibiotic resistance. CONCLUSION: The rate of antibiotic resistance among H. pylori isolates in Lima, Peru, is higher than expected and presents cause for concern. To develop more targeted eradication therapies for H. pylori in Peru, more research is needed to better characterize antibiotic resistance among a larger number of clinical isolates prospectively.	en_US
dc.language.iso	eng
dc.publisher	Dove Medical Press
dc.relation.ispartofseries	Infection and Drug Resistance
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject	Peru	en_US
dc.subject	amoxicillin	en_US
dc.subject	antibiotic resistance	en_US
dc.subject	Helicobacter pylori	en_US
dc.title	Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.2147/IDR.S123798
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.01.05
dc.relation.issn	1178-6973