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Antioxidant and neuroprotector effect of Lepidium meyenii (maca) methanol leaf extract against 6-hydroxy dopamine (6-OHDA)-induced toxicity in PC12 cells

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dc.contributor.author Rodriguez-Huaman, Angel
dc.contributor.author Casimiro-Gonzales, Sandra
dc.contributor.author Chavez-Perez, Jorge Antonio
dc.contributor.author Gonzales-Arimborgo, Carla
dc.contributor.author Cisneros-Fernandez, Richard
dc.contributor.author Aguilar-Mendoza, Luis Angel
dc.contributor.author Gonzales, Gustavo F.
dc.date.accessioned 2019-01-25T15:02:17Z
dc.date.available 2019-01-25T15:02:17Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/20.500.12866/4602
dc.description.abstract Reactive oxygen species (ROS) are normally produced during cell metabolism, there is strong evidence to suggest that ROS produced in excess impair the cell and may be etiologically related to various neurodegenerative diseases. This study was undertaken to examine the effects of Lepidium meyenii (MACA) methanol leaf extract on neurotoxicity in PC12 cell exposed to 6-hydroxydopamine (6-OHDA). Fresh samples of "maca" leaves were processed in order to obtain foliar extracts and to evaluate the neurobiological activity on PC12 cells, subjected to the cytotoxic effect of 6-OHDA through the determination of the capacity antioxidant, cell viability and cytotoxicity assays on PC12 cells. The results of the tests of antioxidant activity, showed maximum values of 2262.37 and 1305.36 expressed in Trolox equivalents (TEAC), for the methanolic and aqueous fractions respectively. Cell viability assays at a dose of 10 mug extract showed an increase of 31% and 60% at 6 and 12 h of pretreatment, respectively. Cytotoxicity assays at the same dose and exposure time showed a 31.4% and 47.8% reduction in lactate dehydrogenase (LDH) activity and an increase in superoxide dismutase (SOD) activity. The results allow us to affirm that the methanolic foliar extract of "maca" presents in vitro neurobiological activity of antioxidant protection, increase in cell viability and reduction of cytotoxicity against oxidative stress generated by 6-OHDA. In conclusion, the present study shows a protective role for Lepidium meyenii leaf extract on 6-OHDA-induced toxicity by an antioxidant effect. en_US
dc.language.iso eng
dc.publisher Taylor & Francis
dc.relation.ispartof urn:issn:1537-6524
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject Lepidium meyenii en_US
dc.subject antioxidant en_US
dc.subject Cell Culture Techniques en_US
dc.subject Dose-Response Relationship, Drug en_US
dc.subject neuroprotector en_US
dc.subject PC12 Cells en_US
dc.subject Rats en_US
dc.subject reactive oxygen species en_US
dc.subject Antioxidants/isolation & purification/pharmacology en_US
dc.subject Cell Survival/drug effects en_US
dc.subject Lepidium/chemistry en_US
dc.subject Methanol/chemistry en_US
dc.subject Neuroprotective Agents/isolation & purification/pharmacology en_US
dc.subject Oxidopamine/toxicity en_US
dc.subject Plant Extracts/isolation & purification/pharmacology en_US
dc.subject Plant Leaves/chemistry en_US
dc.title Antioxidant and neuroprotector effect of Lepidium meyenii (maca) methanol leaf extract against 6-hydroxy dopamine (6-OHDA)-induced toxicity in PC12 cells en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1080/15376516.2016.1275908
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.07


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