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Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods

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dc.contributor.author Silva, Claudia
dc.contributor.author Betancor, Laura
dc.contributor.author García, Coralith
dc.contributor.author Astocondor, Lizeth
dc.contributor.author Hinostroza, Noemí
dc.contributor.author Bisio, Julieta
dc.contributor.author Rivera, Javier
dc.contributor.author Perezgasga, Lucía
dc.contributor.author Pérez Escanda, Victoria
dc.contributor.author Yim, Lucía
dc.contributor.author Jacobs, Jan
dc.contributor.author García-Del Portillo, Francisco
dc.contributor.author SalmoIber CYTED Network
dc.contributor.author Chabalgoity, José A.
dc.contributor.author Puente, José L.
dc.date.accessioned 2019-01-25T15:02:20Z
dc.date.available 2019-01-25T15:02:20Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/20.500.12866/4617
dc.description.abstract In this study, different molecular typing tools were applied to characterize 95 Salmonella enterica blood isolates collected between 2008 and 2013 from patients at nine public hospitals in Lima, Peru. Combined results of multiplex PCR serotyping, two- and seven-loci multilocus sequence typing (MLST) schemes, serotyping, IS200 amplification and RAPD fingerprints, showed that these infections were caused by eight different serovars: Enteritidis, Typhimurium, Typhi, Choleraesuis, Dublin, Paratyphi A, Paratyphi B and Infantis. Among these, Enteritidis, Typhimurium and Typhi were the most prevalent, representing 45, 36 and 11% of the isolates, respectively. Most isolates (74%) were not resistant to ten primarily used antimicrobial drugs; however, 37% of the strains showed intermediate susceptibility to ciprofloxacin (ISC). Antimicrobial resistance integrons were carried by one Dublin (dfra1 and aadA1) and two Infantis (aadA1) isolates. The two Infantis isolates were multidrug resistant and harbored a large megaplasmid. Amplification of spvC and spvRA regions showed that all Enteritidis (n = 42), Typhimurium (n = 34), Choleraesuis (n = 3) and Dublin (n = 1) isolates carried the Salmonella virulence plasmid (pSV). We conclude that the classic serotyping method can be substituted by the multiplex PCR and, when necessary, sequencing of only one or two loci of the MLST scheme is a valuable tool to confirm the results. The effectiveness and feasibility of different typing tools is discussed. en_US
dc.language.iso eng
dc.publisher PLoS
dc.relation.ispartof urn:issn:1932-6203
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject Bacteremia/microbiology en_US
dc.subject Humans en_US
dc.subject Multiplex Polymerase Chain Reaction en_US
dc.subject Salmonella enterica/genetics/isolation & purification en_US
dc.title Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pone.0189946
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01

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