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Development of ELISAs for diagnosis of acute typhoid fever in Nigerian children

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dc.contributor.author Felgner, Jiin
dc.contributor.author Jain, Aarti
dc.contributor.author Nakajima, Rie
dc.contributor.author Liang, Li
dc.contributor.author Jasinskas, Algis
dc.contributor.author Gotuzzo Herencia, José Eduardo
dc.contributor.author Vinetz, Joseph Michael
dc.contributor.author Miyajima, Fabio
dc.contributor.author Pirmohamed, Munir
dc.contributor.author Hassan-Hanga, Fatimah
dc.contributor.author Umoru, Dominic
dc.contributor.author Jibir, Binta Wudil
dc.contributor.author Gambo, Safiya
dc.contributor.author Olateju, Kudirat
dc.contributor.author Felgner, Philip L.
dc.contributor.author Obaro, Stephen
dc.contributor.author Davies, D. Huw
dc.date.accessioned 2019-01-25T15:18:33Z
dc.date.available 2019-01-25T15:18:33Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/20.500.12866/4635
dc.description.abstract Improved serodiagnostic tests for typhoid fever (TF) are needed for surveillance, to facilitate patient management, curb antibiotic resistance, and inform public health programs. To address this need, IgA, IgM and IgG ELISAs using Salmonella enterica serovar Typhi (S. Typhi) lipopolysaccharide (LPS) and hemolysin E (t1477) protein were conducted on 86 Nigerian pediatric TF and 29 non-typhoidal Salmonella (NTS) cases, 178 culture-negative febrile cases, 28 "other" (i.e., non-Salmonella) pediatric infections, and 48 healthy Nigerian children. The best discrimination was achieved between TF and healthy children. LPS-specific IgA and IgM provided receiver operator characteristic areas under the curve (ROC AUC) values of 0.963 and 0.968, respectively, and 0.978 for IgA+M combined. Similar performance was achieved with t1477-specific IgA and IgM (0.968 and 0.968, respectively; 0.976 combined). IgG against LPS and t1477 was less accurate for discriminating these groups, possibly as a consequence of previous exposure, although ROC AUC values were still high (0.928 and 0.932, respectively). Importantly, discrimination between TF and children with other infections was maintained by LPS-specific IgA and IgM (AUC = 0.903 and 0.934, respectively; 0.938 combined), and slightly reduced for IgG (0.909), while t1477-specific IgG performed best (0.914). A similar pattern was seen when comparing TF with other infections from outside Nigeria. The t1477 may be recognized by cross-reactive antibodies from other acute infections, although a robust IgG response may provide some diagnostic utility in populations where incidence of other infections is low, such as in children. The data are consistent with IgA and IgM against S. Typhi LPS being specific markers of acute TF. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Antibodies, Bacterial/blood en_US
dc.subject Child en_US
dc.subject Child, Preschool en_US
dc.subject Enzyme-Linked Immunosorbent Assay/methods en_US
dc.subject Hemolysin Proteins/immunology en_US
dc.subject Humans en_US
dc.subject Immunoglobulin A/blood en_US
dc.subject Immunoglobulin G/blood en_US
dc.subject Immunoglobulin M/blood en_US
dc.subject Infant en_US
dc.subject Lipopolysaccharides/immunology en_US
dc.subject Nigeria en_US
dc.subject ROC Curve en_US
dc.subject Salmonella typhi/immunology en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Serologic Tests/methods en_US
dc.subject Typhoid Fever/diagnosis en_US
dc.title Development of ELISAs for diagnosis of acute typhoid fever in Nigerian children en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0005679
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


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