Universidad Peruana Cayetano Heredia

Molecular and Phenotypic Characterization of Diarrheagenic Escherichia coli Strains Isolated from Bacteremic Children

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dc.contributor.author Riveros Ramirez, Maribel Denise
dc.contributor.author García, Wilfredo
dc.contributor.author García Apac, Coralith Marlinda
dc.contributor.author Durand Vara, David Percy
dc.contributor.author Mercado, Erik
dc.contributor.author Ruiz, Joaquim
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.date.accessioned 2019-01-25T15:28:06Z
dc.date.available 2019-01-25T15:28:06Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/20.500.12866/4704
dc.description.abstract Escherichia coli is an important cause of Gram-negative bacteremia. The aim of this study was to characterize at the molecular and phenotypic levels E. coli strains belonging to different diarrheagenic pathotypes [diarrheagenic E. coli (DEC)] isolated from bacteremia in children younger than 5 years of age. Seventy bacteremia E. coli strains were collected in a prospective study in 12 hospitals in Lima, Peru. The presence of virulence genes associated with DEC [enterotoxigenic (lt and st), enteropathogenic (eaeA), shiga toxin-producing (stx1and stx2), enteroinvasive (ipaH), enteroaggregative (aggR), and diffusely adherent (daaD)] was determined by multiplex real-time polymerase chain reaction (PCR). Those positive E. coli strains were further analyzed for 18 additional virulence factors encoding genes and others phenotypic features. Virulence genes associated with DEC were identified in seven bacteremic children (10%), including: one aggR-positive [enteroaggregative E. coli (EAEC)], one eaeA-positive [enteropathogenic E. coli (EPEC)], one st-positive [enterotoxigenic E. coli (ETEC)], one daaD-positive [diffusely adherent E. coli (DAEC)], and three strain positive for aggR and daaD (EAEC/DAEC) at the same time. All strains, except EPEC, had the Ag43 adhesin, and all, except ETEC had the siderophore gene fyuA. The phylogenetic profile of these strains was variable, two (B2), two (D), two (A), and one (B1) strain. These isolates were susceptible to all tested antibacterial agents except to ampicillin and gentamicin. The three EAEC/DAEC strains showed biofilm formation and aggregative adhesion and had the same repetitive extragenic palindromic-PCR patterns. These findings suggest that some DEC strains, especially agg-R and daa-D positive, might cause bacteremia in children. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Adhesins, Bacterial/genetics/metabolism en_US
dc.subject Ampicillin/therapeutic use en_US
dc.subject Anti-Bacterial Agents/therapeutic use en_US
dc.subject Bacteremia/diagnosis/drug therapy/epidemiology en_US
dc.subject Child, Preschool en_US
dc.subject Diarrhea/drug therapy/epidemiology/microbiology en_US
dc.subject DNA, Bacterial/genetics en_US
dc.subject Drug Resistance, Multiple, Bacterial en_US
dc.subject Enteropathogenic Escherichia coli/drug effects/genetics/isolation & purification en_US
dc.subject Enterotoxigenic Escherichia coli/drug effects/genetics/isolation & purification en_US
dc.subject Humans en_US
dc.subject Infant en_US
dc.subject Infant, Newborn en_US
dc.subject Peru/epidemiology en_US
dc.subject Phylogeny en_US
dc.subject Prospective Studies en_US
dc.subject Real-Time Polymerase Chain Reaction en_US
dc.title Molecular and Phenotypic Characterization of Diarrheagenic Escherichia coli Strains Isolated from Bacteremic Children en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.17-0066
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1476-1645


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