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Polyclonal antibodies for the detection of Trypanosoma cruzi circulating antigens

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dc.contributor.author Málaga-Machaca, Edith S.
dc.contributor.author Romero-Ramirez, Alessandra
dc.contributor.author Gilman, Robert H.
dc.contributor.author Astupiña-Figueroa, Sofía
dc.contributor.author Angulo, Noelia
dc.contributor.author Florentini, Alejandro
dc.contributor.author Lovon-Luque, Cinthya J.
dc.contributor.author Gonza, Remo A.
dc.contributor.author Del Carpio-Sanz, Ada
dc.contributor.author Cabello, Inés
dc.contributor.author Camargo, Rosina
dc.contributor.author Recuenco, Fernando
dc.contributor.author Barrueta-Soria, Liliam A.
dc.contributor.author Verastegui, Manuela R.
dc.contributor.author Calderon, Maritza
dc.contributor.author Mayta, Holger
dc.date.accessioned 2019-01-25T15:28:07Z
dc.date.available 2019-01-25T15:28:07Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/20.500.12866/4725
dc.description.abstract BACKGROUND: Detection of Trypanosoma cruzi antigens in clinical samples is considered an important diagnostic tool for Chagas disease. The production and use of polyclonal antibodies may contribute to an increase in the sensitivity of immunodiagnosis of Chagas disease. METHODOLOGY/PRINCIPAL FINDINGS: Polyclonal antibodies were raised in alpacas, rabbits, and hens immunized with trypomastigote excreted-secreted antigen, membrane proteins, trypomastigote lysate antigen and recombinant 1F8 to produce polyclonal antibodies. Western blot analysis was performed to determine specificity of the developed antibodies. An antigen capture ELISA of circulating antigens in serum, plasma and urine samples was developed using IgY polyclonal antibodies against T. cruzi membrane antigens (capture antibody) and IgG from alpaca raised against TESA. A total of 33 serum, 23 plasma and 9 urine samples were analyzed using the developed test. Among serum samples, compared to serology, the antigen capture ELISA tested positive in 55% of samples. All plasma samples from serology positive subjects were positive in the antigen capture ELISA. All urine positive samples had corresponding plasma samples that were also positive when tested by the antigen capture ELISA. CONCLUSIONS: Polyclonal antibodies are useful for detection of circulating antigens in both the plasma and urine of infected individuals. Detection of antigens is direct evidence of the presence of the parasite, and could be a better surrogate of current infection status. en_US
dc.language.iso eng
dc.publisher PLoS
dc.relation.ispartof urn:issn:1935-2735
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject Antibodies, Protozoan/immunology en_US
dc.subject Antigens, Protozoan/blood/urine en_US
dc.subject Camelids, New World en_US
dc.subject Chagas Disease/diagnosis en_US
dc.subject Chickens en_US
dc.subject Enzyme-Linked Immunosorbent Assay/methods en_US
dc.subject Rabbits en_US
dc.subject Serologic Tests/methods en_US
dc.subject Trypanosoma cruzi/immunology en_US
dc.title Polyclonal antibodies for the detection of Trypanosoma cruzi circulating antigens en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0006069
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06

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