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Recombinase Polymerase Amplification Compared to Real-Time Polymerase Chain Reaction Test for the Detection of Fasciola hepatica in Human Stool

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dc.contributor.author Cabada, Miguel M.
dc.contributor.author Malaga, Jose L.
dc.contributor.author Castellanos-Gonzalez, Alejandro
dc.contributor.author Bagwell, Kelli A.
dc.contributor.author Naeger, Patrick A.
dc.contributor.author Rogers, Hayley K.
dc.contributor.author Maharsi, Safa
dc.contributor.author Mbaka, Maryann
dc.contributor.author White, A. Clinton Jr
dc.date.accessioned 2019-01-25T16:03:19Z
dc.date.available 2019-01-25T16:03:19Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/20.500.12866/4742
dc.description.abstract Fasciola hepatica is the most widely distributed trematode infection in the world. Control efforts may be hindered by the lack of diagnostic capacity especially in remote endemic areas. Polymerase chain reaction (PCR)-based methods offer high sensitivity and specificity but require expensive technology. However, the recombinase polymerase amplification (RPA) is an efficient isothermal method that eliminates the need for a thermal cycler and has a high deployment potential to resource-limited settings. We report on the characterization of RPA and PCR tests to detect Fasciola infection in clinical stool samples with low egg burdens. The sensitivity of the RPA and PCR were 87% and 66%, respectively. Both tests were 100% specific showing no cross-reactivity with trematode, cestode, or nematode parasites. In addition, RPA and PCR were able to detect 47% and 26% of infections not detected by microscopy, respectively. The RPA adapted to a lateral flow platform was more sensitive than gel-based detection of the reaction products. In conclusion, the Fasciola RPA is a highly sensitive and specific test to diagnose chronic infection using stool samples. The Fasciola RPA lateral flow has the potential for deployment to endemic areas after further characterization. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Microscopy en_US
dc.subject Animals en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Nucleic Acid Amplification Techniques/methods en_US
dc.subject Fasciola hepatica/genetics en_US
dc.subject Fascioliasis/diagnosis/parasitology en_US
dc.subject Feces/parasitology en_US
dc.subject Real-Time Polymerase Chain Reaction/methods en_US
dc.title Recombinase Polymerase Amplification Compared to Real-Time Polymerase Chain Reaction Test for the Detection of Fasciola hepatica in Human Stool en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.16-0601
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1476-1645


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