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“Inhibiting inhibitors”: Preliminary results of a new “DNA extraction-amplification” disinhibition technique in critical human samples

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dc.contributor.author Gomes, C.
dc.contributor.author Alonso, A.
dc.contributor.author Marquina, D.
dc.contributor.author Guardià, M.
dc.contributor.author López-Matayoshi, C.
dc.contributor.author Palomo-Díez, S.
dc.contributor.author Perea-Peréz, B.
dc.contributor.author Gibaja, J. F.
dc.contributor.author Arroyo-Pardo, E.
dc.date.accessioned 2019-01-25T16:20:57Z
dc.date.available 2019-01-25T16:20:57Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/20.500.12866/4806
dc.description.abstract The present study focused on the genetic analysis of a presumptive Bronze Age family burial, with at least six skeletons (Plana Castell, Barcelona, Spain). In order to investigate the individuals’ biological affiliation, a molecular study was undertaken, but it was impossible to obtain a positive DNA amplification. The excavation data indicated that the skeletons were buried in an old location used as a dumping ground, being very likely the presence of biological and/or chemical contaminants. To overcome this difficulty, we undertook several modifications both in the extraction as in the amplification protocol, like MgCl2 concentration, or the inclusion of Bovine Serum Albumin (BSA). Despite their antiquity, the preliminary results indicate that all samples were effectively inhibited, and for the first time we were able to obtain mtDNA results, specifically, for the Hypervariable mitochondrial region 1. It also allowed us to infer that our protocol performance increased considerable, mainly the amplification modifications. In general, the best results were obtained with MgCl2 0,6 μL (20 mM) and BSA 2 mg/mL per sample. Our result is quite important in a forensic analysis, mainly in cases where corpse/s is/are buried directly in the soil. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof urn:issn:0379-0738
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject aluminum oxide en_US
dc.subject Antiquity en_US
dc.subject Article en_US
dc.subject bovine serum albumin en_US
dc.subject Bronze age en_US
dc.subject BSA en_US
dc.subject controlled study en_US
dc.subject DNA degradation en_US
dc.subject DNA extraction en_US
dc.subject DNA inhibition en_US
dc.subject forensic genetics en_US
dc.subject gene amplification en_US
dc.subject human en_US
dc.subject magnesium chloride en_US
dc.subject MgCl2 en_US
dc.subject mitochondrial DNA en_US
dc.subject pH en_US
dc.subject priority journal en_US
dc.title “Inhibiting inhibitors”: Preliminary results of a new “DNA extraction-amplification” disinhibition technique in critical human samples en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.fsigss.2017.09.066

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