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Influence of Leishmania RNA Virus 1 on Proinflammatory Biomarker Expression in a Human Macrophage Model of American Tegumentary Leishmaniasis

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dc.contributor.author Kariyawasam, R.
dc.contributor.author Grewal, J.
dc.contributor.author Lau, R.
dc.contributor.author Purssell, A.
dc.contributor.author Valencia, B. M.
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Boggild, A. K.
dc.date.accessioned 2019-01-25T16:36:28Z
dc.date.available 2019-01-25T16:36:28Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/20.500.12866/4841
dc.description.abstract Backgound. Species of the Leishmania Viannia (L. V.) subgenus harbor the double-stranded Leishmania RNA virus 1 (LRV-1), previously identified in isolates from Brazil and Peru. Higher levels of LRV-1 in metastasizing strains of L. V. guyanensis have been documented in both human and murine models, and correlated to disease severity. Methods. Expression of proinflammatory biomarkers, including interleukin (IL) 1 beta, tumor necrosis factor alpha (TNF-alpha), CXCL10, CCL5, IL-6, and superoxide dismutase, in human macrophages infected with 3 ATCC and 5 clinical isolates of L. V. braziliensis, L. V. guyanensis, and L. V. panamensis for 24 and 48 hours were measured by commercial enzyme immunoassay. Analyses were performed at 24 and 48 hours, stratified by LRV-1 status and species. Results. LRV-1-positive L. V. braziliensis demonstrated significantly lower expression levels of TNF-alpha (P =.01), IL-1 beta (P =.0015), IL-6 (P =.001), and CXCL10 (P =.0004) compared with LRV-1-negative L. V. braziliensis. No differences were observed in strains of L. V. panamensis by LRV-1 status. Conclusions. Compared to LRV-1-negative L. V. braziliensis, LRV-1-positive strains of L. V. braziliensis produced a predominant Th2-biased immune response, correlated in humans to poorer immunologic control of infection and more severe disease, including mucosal leishmaniasis. Effects of LRV-1 on the pathogenesis of American tegumentary leishmaniasis may be species specific. en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartofseries Journal of Infectious Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject American tegumentary leishmaniasis en_US
dc.subject diagnosis en_US
dc.subject cytokines en_US
dc.subject immunophenotype en_US
dc.subject Leishmania (Viannia) braziliensis en_US
dc.subject Leishmania RNA virus 1 en_US
dc.subject identification en_US
dc.subject Infectious Diseases en_US
dc.subject Immunology en_US
dc.subject Microbiology en_US
dc.subject survival en_US
dc.subject cutaneous leishmaniasis en_US
dc.subject immune-response en_US
dc.subject leishmaniasis en_US
dc.subject mucocutaneous leishmaniasis en_US
dc.subject mucosal en_US
dc.subject new-world en_US
dc.title Influence of Leishmania RNA Virus 1 on Proinflammatory Biomarker Expression in a Human Macrophage Model of American Tegumentary Leishmaniasis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1093/infdis/jix416
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 1537-6613


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