Diarrheagenic Escherichia coli: Prevalence and Pathotype Distribution in Children from Peruvian Rural Communities

Arango, Sara; Vigo, Natalia-I.; Zambruni, Mara; Durand Vara, David Percy; Riveros Ramirez, Maribel Denise; Ochoa Woodell, Theresa Jean; Acosta, Gonzalo-J.

dc.contributor.author	Acosta, Gonzalo-J.
dc.contributor.author	Vigo, Natalia-I.
dc.contributor.author	Durand Vara, David Percy
dc.contributor.author	Riveros Ramirez, Maribel Denise
dc.contributor.author	Arango, Sara
dc.contributor.author	Zambruni, Mara
dc.contributor.author	Ochoa Woodell, Theresa Jean
dc.date.accessioned	2019-02-06T14:45:08Z
dc.date.available	2019-02-06T14:45:08Z
dc.date.issued	2016
dc.identifier.uri	https://hdl.handle.net/20.500.12866/5015
dc.description.abstract	Diarrheagenic Escherichia coli (DEC) are common pathogens of childhood gastrointestinal infections worldwide. To date, research tracking DEC has mainly been completed in urban areas. This study aims to determine the prevalence and pathotype distribution of DEC strains in children from rural Peruvian communities and to establish their association with malnutrition. In this prospective cohort, 93 children aged 6-13 months from rural communities of Urubamba (Andes) and Moyobamba (jungle) were followed for 6 months. Diarrheal and control stool samples were analyzed using multiplex real-time polymerase chain reaction to identify the presence of virulence genes of DEC strains. The overall isolation rate of DEC was 43.0% (352/820). Enteroaggregative E. coli (EAEC, 20.4%), enteropathogenic E. coli (EPEC, 14.2%), and diffusely aggregative E. coli (DAEC, 11.0%) were the most prevalent pathotypes. EAEC was more frequently found in Moyobamba samples (P < 0.01). EPEC was the only strain significantly more frequent in diarrheal than asymptomatic control samples (P < 0.01). DEC strains were more prevalent among younger children (aged 6-12 months, P < 0.05). A decline in height-for-age Z-score (HAZ) was observed in 75.7% of children overall. EAEC was more frequently isolated among children who had a greater HAZ decline (P < 0.05). In conclusion, DEC strains were frequently found in stool samples from children in rural communities of the highlands and jungle of Peru. In addition, children with a greater decline in their growth rate had higher EAEC isolation rates, highlighting the importance of this pathogen in child malnutrition.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Enteropathogenic, Escherichia coli	en_US
dc.subject	Diarrhea/epidemiology/etiology/microbiology	en_US
dc.subject	Escherichia coli Infections/epidemiology/microbiology	en_US
dc.subject	Female	en_US
dc.subject	Humans	en_US
dc.subject	Infant	en_US
dc.subject	Male	en_US
dc.subject	Peru/epidemiology	en_US
dc.subject	Prevalence	en_US
dc.subject	Rural Population/statistics & numerical data	en_US
dc.title	Diarrheagenic Escherichia coli: Prevalence and Pathotype Distribution in Children from Peruvian Rural Communities	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.16-0220
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645