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Colorimetric Detection of Plasmodium vivax in Urine Using MSP10 Oligonucleotides and Gold Nanoparticles

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dc.contributor.author Alnasser, Yossef
dc.contributor.author Ferradas, Cusi
dc.contributor.author Clark, Taryn
dc.contributor.author Calderon, Maritza
dc.contributor.author Gurbillon, Alejandro
dc.contributor.author Gamboa, Dionicia
dc.contributor.author McKakpo, Uri S.
dc.contributor.author Quakyi, Isabella A.
dc.contributor.author Bosompem, Kwabena M.
dc.contributor.author Sullivan, David J.
dc.contributor.author Vinetz, Joseph M.
dc.contributor.author Gilman, Robert H.
dc.date.accessioned 2019-02-06T14:45:09Z
dc.date.available 2019-02-06T14:45:09Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5019
dc.description.abstract Plasmodium vivax is the most prevalent cause of human malaria in the world and can lead to severe disease with high potential for relapse. Its genetic and geographic diversities make it challenging to control. P. vivax is understudied and to achieve control of malaria in endemic areas, a rapid, accurate, and simple diagnostic tool is necessary. In this pilot study, we found that a colorimetric system using AuNPs and MSP10 DNA detection in urine can provide fast, easy, and inexpensive identification of P. vivax. The test exhibited promising sensitivity (84%), high specificity (97%), and only mild cross-reactivity with P. falciparum (21%). It is simple to use, with a visible color change that negates the need for a spectrometer, making it suitable for use in austere conditions. Using urine eliminates the need for finger-prick, increasing both the safety profile and patient acceptance of this model. en_US
dc.language.iso eng
dc.publisher PLoS
dc.relation.ispartof urn:issn:1935-2735
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Metal Nanoparticles en_US
dc.subject Oligonucleotides en_US
dc.subject Antigens, Protozoan/genetics en_US
dc.subject Colorimetry/economics/methods/standards en_US
dc.subject Cross Reactions en_US
dc.subject DNA, Protozoan/urine en_US
dc.subject Gold en_US
dc.subject Humans en_US
dc.subject Malaria, Vivax/diagnosis/parasitology/urine en_US
dc.subject Mass Screening en_US
dc.subject Microscopy en_US
dc.subject Parasitemia/diagnosis/parasitology en_US
dc.subject Pilot Projects en_US
dc.subject Plasmodium vivax/genetics/isolation & purification/ultrastructure en_US
dc.subject Protozoan Proteins/genetics en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Urine/parasitology en_US
dc.title Colorimetric Detection of Plasmodium vivax in Urine Using MSP10 Oligonucleotides and Gold Nanoparticles en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0005029
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06

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