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Perilesional Inflammation in Neurocysticercosis - Relationship Between Contrast-Enhanced Magnetic Resonance Imaging, Evans Blue Staining and Histopathology in the Pig Model

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dc.contributor.author Cangalaya, Carla
dc.contributor.author Bustos, Javier-A.
dc.contributor.author Calcina, Juan
dc.contributor.author Vargas-Calla, Ana
dc.contributor.author Suarez, Diego
dc.contributor.author Gonzalez, Armando-E.
dc.contributor.author Chacaltana, Juan
dc.contributor.author Guerra-Giraldez, Cristina
dc.contributor.author Mahanty, Siddhartha
dc.contributor.author Nash, Theodore-E.
dc.contributor.author Garcia, Hector-H.
dc.date.accessioned 2019-02-06T14:45:11Z
dc.date.available 2019-02-06T14:45:11Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5037
dc.description.abstract BACKGROUND: Disease manifestations in neurocysticercosis (NCC) are frequently due to inflammation of degenerating Taenia solium brain cysts. Exacerbated inflammation post anthelmintic treatment is associated with leakage of the blood brain barrier (BBB) using Evans blue (EB) staining. How well EB extravasation into the brain correlates with magnetic resonance imaging (MRI) using gadolinium (Gd) enhancement as a contrast agent and pericystic inflammation was analyzed in pigs harboring brain cysts of Taenia solium. METHODOLOGY/PRINCIPAL FINDINGS: Three groups of 4 naturally infected pigs were assessed. The first and second groups were treated with both praziquantel plus albendazole and sacrificed two and five days post treatment, respectively. A third untreated group remained untreated. Pigs were injected with EB two hours prior to evaluation by Gd-enhanced T1-MRI, and euthanized. The EB staining for each cyst capsule was scored (EB grades were 0: 0%; 1: up to 50%; 2: over 50% but less than 100%; 3: 100%). Similarly, the Gd enhancement around each cyst was qualitatively and quantitatively scored from the MRI. The extent of pericystic inflammation on histology was scored in increasing severity as IS1, IS2, IS3 and IS4. Grade 3 EB staining and enhancement was only seen in treated capsules. Also, treated groups had higher Gd intensity than the untreated group. Grades of enhancement correlated significantly with Gd enhancement intensity. EB staining was correlated with Gd enhancement intensity and with IS4 in the treated groups. These correlations were stronger in internally located cysts compared to superficial cysts in treated groups. SIGNIFICANCE: EB staining and Gd enhancement strongly correlate. The intensity of enhancement determined by MRI is a good indication of the degree of inflammation. Similarly, EB staining highly correlates with the degree of inflammation and may be applied to study inflammation in the pig model of NCC. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartof urn:issn:1935-2735
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject Brain/pathology en_US
dc.subject Disease Models, Animal en_US
dc.subject Evans Blue/chemistry en_US
dc.subject Histology en_US
dc.subject Humans en_US
dc.subject Magnetic Resonance Imaging/methods en_US
dc.subject Neurocysticercosis/diagnostic imaging/immunology/pathology en_US
dc.subject Staining and Labeling/methods en_US
dc.subject Swine en_US
dc.title Perilesional Inflammation in Neurocysticercosis - Relationship Between Contrast-Enhanced Magnetic Resonance Imaging, Evans Blue Staining and Histopathology in the Pig Model en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0004869
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE


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