Universidad Peruana Cayetano Heredia

Nanoparticle-Based Histidine-Rich Protein-2 Assay for the Detection of the Malaria Parasite Plasmodium falciparum

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dc.contributor.author Castro-Sesquen, Yagahira E.
dc.contributor.author Kim, Chloe
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Sullivan, David J.
dc.contributor.author Searson, Peter C.
dc.date.accessioned 2019-02-06T14:45:11Z
dc.date.available 2019-02-06T14:45:11Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5042
dc.description.abstract A nanoparticle-based assay for detection and quantification of Plasmodium falciparum histidine-rich protein 2 (HRP2) in urine and serum is reported. The assay uses magnetic beads conjugated with anti-HRP2 antibody for protein capture and concentration, and antibody-conjugated quantum dots for optical detection. Western blot analysis demonstrated that magnetic beads allow the concentration of HRP2 protein in urine by 20-fold. The concentration effect was achieved because large volume of urine can be incubated with beads, and magnetic separation can be easily performed in minutes to isolate beads containing HRP2 protein. Magnetic beads and quantum dots conjugated to anti-HRP2 antibodies allows the detection of low concentrations of HRP2 protein (0.5 ng/mL), and quantification in the range of 33-2,000 ng/mL corresponding to the range associated with non-severe to severe malaria. This assay can be easily adapted to a noninvasive point-of-care test for classification of severe malaria. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Biological Assay en_US
dc.subject Antibodies, Protozoan/chemistry en_US
dc.subject Antigens, Protozoan/urine en_US
dc.subject Blotting, Western en_US
dc.subject Calibration en_US
dc.subject Gene Expression en_US
dc.subject Humans en_US
dc.subject Magnets en_US
dc.subject Malaria, Falciparum/diagnosis/parasitology/urine en_US
dc.subject Nanoparticles/chemistry en_US
dc.subject Plasmodium falciparum/genetics/isolation & purification en_US
dc.subject Protozoan Proteins/urine en_US
dc.subject Quantum Dots/chemistry en_US
dc.subject Sensitivity and Specificity en_US
dc.title Nanoparticle-Based Histidine-Rich Protein-2 Assay for the Detection of the Malaria Parasite Plasmodium falciparum en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.15-0772
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1476-1645


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