Nanoparticle-Based Histidine-Rich Protein-2 Assay for the Detection of the Malaria Parasite Plasmodium falciparum

Castro-Sesquen, Yagahira E.; Kim, Chloe; Gilman, Robert Hugh; Sullivan, David J.; Searson, Peter C.

dc.contributor.author	Castro-Sesquen, Yagahira E.
dc.contributor.author	Kim, Chloe
dc.contributor.author	Gilman, Robert Hugh
dc.contributor.author	Sullivan, David J.
dc.contributor.author	Searson, Peter C.
dc.date.accessioned	2019-02-06T14:45:11Z
dc.date.available	2019-02-06T14:45:11Z
dc.date.issued	2016
dc.identifier.uri	https://hdl.handle.net/20.500.12866/5042
dc.description.abstract	A nanoparticle-based assay for detection and quantification of Plasmodium falciparum histidine-rich protein 2 (HRP2) in urine and serum is reported. The assay uses magnetic beads conjugated with anti-HRP2 antibody for protein capture and concentration, and antibody-conjugated quantum dots for optical detection. Western blot analysis demonstrated that magnetic beads allow the concentration of HRP2 protein in urine by 20-fold. The concentration effect was achieved because large volume of urine can be incubated with beads, and magnetic separation can be easily performed in minutes to isolate beads containing HRP2 protein. Magnetic beads and quantum dots conjugated to anti-HRP2 antibodies allows the detection of low concentrations of HRP2 protein (0.5 ng/mL), and quantification in the range of 33-2,000 ng/mL corresponding to the range associated with non-severe to severe malaria. This assay can be easily adapted to a noninvasive point-of-care test for classification of severe malaria.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Biological Assay	en_US
dc.subject	Antibodies, Protozoan/chemistry	en_US
dc.subject	Antigens, Protozoan/urine	en_US
dc.subject	Blotting, Western	en_US
dc.subject	Calibration	en_US
dc.subject	Gene Expression	en_US
dc.subject	Humans	en_US
dc.subject	Magnets	en_US
dc.subject	Malaria, Falciparum/diagnosis/parasitology/urine	en_US
dc.subject	Nanoparticles/chemistry	en_US
dc.subject	Plasmodium falciparum/genetics/isolation & purification	en_US
dc.subject	Protozoan Proteins/urine	en_US
dc.subject	Quantum Dots/chemistry	en_US
dc.subject	Sensitivity and Specificity	en_US
dc.title	Nanoparticle-Based Histidine-Rich Protein-2 Assay for the Detection of the Malaria Parasite Plasmodium falciparum	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.15-0772
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645