In Vitro Study of Taenia solium Postoncospheral Form

Chile Andrade, Nancy; Clark, Taryn; Gomez-Puerta, Luis A.; García Lescano, Héctor Hugo; Gavidia, Cesar M.; Palma, Sandra; Gilman, Robert Hugh; Kosek, Jon C.; Arana, Yanina; Ortega, Ynes R.; Verastegui Pimentel, Manuela Renee; Mejia, Alan; Kosek, Margaret; Angulo, Noelia

dc.contributor.author	Chile Andrade, Nancy
dc.contributor.author	Clark, Taryn
dc.contributor.author	Arana, Yanina
dc.contributor.author	Ortega, Ynes R.
dc.contributor.author	Palma, Sandra
dc.contributor.author	Mejia, Alan
dc.contributor.author	Angulo, Noelia
dc.contributor.author	Kosek, Jon C.
dc.contributor.author	Kosek, Margaret
dc.contributor.author	Gomez-Puerta, Luis A.
dc.contributor.author	García Lescano, Héctor Hugo
dc.contributor.author	Gavidia, Cesar M.
dc.contributor.author	Gilman, Robert Hugh
dc.contributor.author	Verastegui Pimentel, Manuela Renee
dc.date.accessioned	2019-02-06T14:45:12Z
dc.date.available	2019-02-06T14:45:12Z
dc.date.issued	2016
dc.identifier.uri	https://hdl.handle.net/20.500.12866/5045
dc.description.abstract	BACKGROUND: The transitional period between the oncosphere and the cysticercus of Taenia solium is the postoncospheral (PO) form, which has not yet been completely characterized. The aim of this work was to standardize a method to obtain T. solium PO forms by in vitro cultivation. We studied the morphology of the PO form and compared the expression of antigenic proteins among the PO form, oncosphere, and cysticerci stages. METHODOLOGY/PRINCIPAL FINDINGS: T. solium activated oncospheres were co-cultured with ten cell lines to obtain PO forms, which we studied at three stages of development--days 15, 30, and 60. A high percentage (32%) of PO forms was obtained using HCT-8 cells in comparison to the other cell lines. The morphology was observed by bright field, scanning, and transmission electron microscopy. Morphology of the PO form changed over time, with the six hooks commonly seen in the oncosphere stage disappearing in the PO forms, and vesicles and microtriches observed in the tegument. The PO forms grew as they aged, reaching a diameter of 2.5 mm at 60 days of culture. 15-30 day PO forms developed into mature cysticerci when inoculated into rats. Antigenic proteins expressed in the PO forms are also expressed by the oncosphere and cysticerci stages, with more cysticerci antigenic proteins expressed as the PO forms ages. CONCLUSIONS/SIGNIFICANCE: This is the first report of an in vitro production method of T. solium PO forms. The changes observed in protein expression may be useful in identifying new targets for vaccine development. In vitro culture of PO form will aid in understanding the host-parasite relationship, since the structural changes of the developing PO forms may reflect the parasite's immunoprotective mechanisms. A wider application of this method could significantly reduce the use of animals, and thus the costs and time required for further experimental investigations.	en_US
dc.language.iso	eng
dc.publisher	Public Library of Science
dc.relation.ispartofseries	PLoS Neglected Tropical Diseases
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject	Animals	en_US
dc.subject	Antigens, Helminth/analysis	en_US
dc.subject	Blotting, Western	en_US
dc.subject	Cell Line	en_US
dc.subject	Coculture Techniques	en_US
dc.subject	Gene Expression Profiling	en_US
dc.subject	Humans	en_US
dc.subject	Microscopy	en_US
dc.subject	Taenia solium/anatomy & histology/genetics/growth & development	en_US
dc.title	In Vitro Study of Taenia solium Postoncospheral Form	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1371/journal.pntd.0004396
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1935-2735