Universidad Peruana Cayetano Heredia

In Vitro Study of Taenia solium Postoncospheral Form

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dc.contributor.author Chile Andrade, Nancy
dc.contributor.author Clark, Taryn
dc.contributor.author Arana, Yanina
dc.contributor.author Ortega, Ynes R.
dc.contributor.author Palma, Sandra
dc.contributor.author Mejia, Alan
dc.contributor.author Angulo, Noelia
dc.contributor.author Kosek, Jon C.
dc.contributor.author Kosek, Margaret
dc.contributor.author Gomez-Puerta, Luis A.
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Gavidia, Cesar M.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.date.accessioned 2019-02-06T14:45:12Z
dc.date.available 2019-02-06T14:45:12Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5045
dc.description.abstract BACKGROUND: The transitional period between the oncosphere and the cysticercus of Taenia solium is the postoncospheral (PO) form, which has not yet been completely characterized. The aim of this work was to standardize a method to obtain T. solium PO forms by in vitro cultivation. We studied the morphology of the PO form and compared the expression of antigenic proteins among the PO form, oncosphere, and cysticerci stages. METHODOLOGY/PRINCIPAL FINDINGS: T. solium activated oncospheres were co-cultured with ten cell lines to obtain PO forms, which we studied at three stages of development--days 15, 30, and 60. A high percentage (32%) of PO forms was obtained using HCT-8 cells in comparison to the other cell lines. The morphology was observed by bright field, scanning, and transmission electron microscopy. Morphology of the PO form changed over time, with the six hooks commonly seen in the oncosphere stage disappearing in the PO forms, and vesicles and microtriches observed in the tegument. The PO forms grew as they aged, reaching a diameter of 2.5 mm at 60 days of culture. 15-30 day PO forms developed into mature cysticerci when inoculated into rats. Antigenic proteins expressed in the PO forms are also expressed by the oncosphere and cysticerci stages, with more cysticerci antigenic proteins expressed as the PO forms ages. CONCLUSIONS/SIGNIFICANCE: This is the first report of an in vitro production method of T. solium PO forms. The changes observed in protein expression may be useful in identifying new targets for vaccine development. In vitro culture of PO form will aid in understanding the host-parasite relationship, since the structural changes of the developing PO forms may reflect the parasite's immunoprotective mechanisms. A wider application of this method could significantly reduce the use of animals, and thus the costs and time required for further experimental investigations. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject Antigens, Helminth/analysis en_US
dc.subject Blotting, Western en_US
dc.subject Cell Line en_US
dc.subject Coculture Techniques en_US
dc.subject Gene Expression Profiling en_US
dc.subject Humans en_US
dc.subject Microscopy en_US
dc.subject Taenia solium/anatomy & histology/genetics/growth & development en_US
dc.title In Vitro Study of Taenia solium Postoncospheral Form en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0004396
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


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