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In Vitro Cytotoxicity Evaluation of Three Root-End Filling Materials in Human Periodontal Ligament Fibroblasts

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dc.contributor.author Coaguila-Llerena, Hernan
dc.contributor.author Vaisberg Wollach, Abraham Jaime
dc.contributor.author Velasquez-Huaman, Zulema
dc.date.accessioned 2019-02-06T14:45:12Z
dc.date.available 2019-02-06T14:45:12Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5047
dc.description.abstract The aim of this study was to evaluate in vitro the cytotoxicity on human periodontal ligament fibroblasts of three root-end filling materials: MTA Angelus(R), EndoSequence Root Repair Material Putty(R) and Super EBA(R). A primary culture of human periodontal ligament fibroblasts was previously obtained in order to evaluate the cytotoxicity of the three extracts from the root-end filling materials after 2 and 7 days of setting. Serial dilutions of these extracts (1:1, 1:2, 1:4 and 1:8) were evaluated at 1, 3 and 7 days using the methyl-thiazol-tetrazolium (MTT) colorimetric assay. Cell viability was evaluated as percentage of the negative control group, which represented 100% cell viability. Statistical analyses were done with t-test, ANOVA and Kruskal-Wallis test at a significance level of 5%. It was found that the main difference among root-end filling materials was in the higher dilutions (p<0.05), but there was a similar behavior in lower dilutions (p>0.05). Cell viability of MTA Angelus(R) was superior for 2-day setting (p<0.05), compared with the other two root-end fillings. There were no statistically significant differences between 7-day set MTA Angelus(R) and EndoSequence Root Repair Material Putty(R). Super EBA(R) showed the lowest percentage of cell viability at higher dilutions (p<0.05). Therefore, MTA Angelus(R) and EndoSequence Root Repair Material Putty(R) were less cytotoxic in the highest dilution (1:1) compared with Super EBA(R). en_US
dc.language.iso eng
dc.publisher Fundação Odontológica de Ribeirão Preto
dc.relation.ispartofseries Brazilian Dental Journal
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Root Canal Filling Materials en_US
dc.subject Cells, Cultured en_US
dc.subject Humans en_US
dc.subject In Vitro Techniques en_US
dc.subject Periodontal Ligament/pathology en_US
dc.title In Vitro Cytotoxicity Evaluation of Three Root-End Filling Materials in Human Periodontal Ligament Fibroblasts en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1590/0103-6440201600447
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.14
dc.relation.issn 1806-4760


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