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Hypothetical granulin-like molecule from Fasciola hepatica identified by bioinformatics analysis

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dc.contributor.author Machicado, Claudia
dc.contributor.author Marcos, Luis-A.
dc.contributor.author Zimic, Mirko
dc.date.accessioned 2019-02-06T14:45:37Z
dc.date.available 2019-02-06T14:45:37Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5111
dc.description.abstract Fasciola hepatica is considered an emergent human pathogen, causing liver fibrosis or cirrhosis, conditions that are known to be direct causes of cancer. Some parasites have been categorized by WHO as carcinogenic agents such as Opisthorchis viverrini, a relative of F. hepatica. Although these two parasites are from the same class (Trematoda), the role of F. hepatica in carcinogenesis is unclear. We hypothesized that F. hepatica might share some features with O. viverrini and to be responsible to induce proliferation of host cells. We analyzed the recently released genome of F. hepatica looking for a gene coding a granulin-like growth factor, a protein secreted by O. viverrini (Ov-GRN-1), which is a potent stimulator of proliferation of host cells. Using computational biology tools, we identified a granulin-like molecule in F. hepatica, here termed FhGLM, which has high sequence identity level to Ov-GRN-1 and human progranulin. We found evidence of an upstream promoter compatible with the expression of FhGLM. The FhGLM architecture showed to have five granulin domains, one of them, the domain 3, was homologue to Ov-GRN-1 and human GRNC. The structure of the FhGLM granulin domain 3 resulted to have the overall folding of its homologue the human GRNC. Our findings show the presence of a homologue of a potent modulator of cell growth in F. hepatica that might have, as other granulins, a proliferative action on host cells during fascioliasis. Future experimental assays to demonstrate the presence of FhGLM in F. hepatica are needed to confirm our hypothesis. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof urn:issn:2193-1801
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Carcinogenesis en_US
dc.subject Fasciola en_US
dc.subject Granulin en_US
dc.subject Helminth en_US
dc.subject Homology en_US
dc.subject Proliferation en_US
dc.title Hypothetical granulin-like molecule from Fasciola hepatica identified by bioinformatics analysis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1186/s40064-016-2443-7
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07


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