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Pathogenicity Island O-122 in enteropathogenic Escherichia coli strains is associated with diarrhea severity in children from Lima Peru

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dc.contributor.author Mercado, Erik-H.
dc.contributor.author Piscoche, Cristian
dc.contributor.author Contreras, Carmen
dc.contributor.author Durand, David
dc.contributor.author Riveros, Maribel
dc.contributor.author Ruiz, Joaquim
dc.contributor.author Ochoa, Theresa-J.
dc.date.accessioned 2019-02-06T14:45:55Z
dc.date.available 2019-02-06T14:45:55Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5121
dc.description.abstract EPEC is an attaching and effacing diarrheal pathogen that carries a large pathogenicity island, locus for enterocyte effacement (LEE). Recently, the pathogenicity island PAI O-122 was described among non-LEE effectors and found to be associated with diarrhea among atypical EPEC strains. It is unknown if incomplete PAI O-122 could be associated with diarrhea duration and severity. To identify these virulence determinants we analyzed 379 EPEC strains isolated from Peruvian children. EPEC was diagnosed by PCR(eae+, stx-) and classified as typical(t-EPEC) or atypical(a-EPEC). To characterize PAI O-122 we amplified three modules by PCR: Module 1(pagC), Module 2(senA, nleB and nleE) and Module 3(lifA/efa-1). To characterize the large ORF lifA/efa-1 we amplified the regions known as efa-N, efa-M and efa-C. Clinical information was obtained from the cohort study. A total of 379 EPEC strains were able to analyze PAI O-122 genes, 128 (10.4%) EPEC strains were isolated from 1235 diarrhea episodes and 251(9.2%) from 2734 healthy controls. t-EPEC strains were isolated from 14.8% (19/128) of children with diarrhea and 25/251(10.0%) from healthy controls. The most frequent PAI O-122 genes were nleE(37.7%), senA(34.6%) and nleB(37.5%), with similar prevalence among diarrhea and control samples. However, lifA/efa-1 was more common among diarrhea cases than healthy control cases (30.5% vs. 21.1%, p<0.05). The presence of complete PAI O-122 was associated with diarrhea episodes of higher severity among single pathogen infection (33.3% vs. 1.8%, p<0.05) mainly due to the presence of a complete lifA/efa-1 gene. In summary, the gene lifA/efa-1 is significantly associated with diarrheal episodes of higher severity, suggesting to be an important virulent factor. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof urn:issn:1618-0607
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Genomic Islands en_US
dc.subject Animals en_US
dc.subject Child, Preschool en_US
dc.subject Children en_US
dc.subject Diarrhea severity en_US
dc.subject Diarrhea/microbiology/pathology en_US
dc.subject DNA, Bacterial/genetics en_US
dc.subject Enteropathogenic escherichia coli en_US
dc.subject Enteropathogenic Escherichia coli/genetics/pathogenicity en_US
dc.subject Escherichia coli Infections/microbiology/pathology en_US
dc.subject Female en_US
dc.subject Genes, Bacterial en_US
dc.subject Humans en_US
dc.subject Infant en_US
dc.subject Male en_US
dc.subject Pathogenicity island O-122 en_US
dc.subject Peru en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject Severity of Illness Index en_US
dc.title Pathogenicity Island O-122 in enteropathogenic Escherichia coli strains is associated with diarrhea severity in children from Lima Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.ijmm.2016.05.005
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE

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