Universidad Peruana Cayetano Heredia
Pathogenicity Island O-122 in enteropathogenic Escherichia coli strains is associated with diarrhea severity in children from Lima Peru
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| dc.contributor.author | Mercado, Erik-H. | |
| dc.contributor.author | Piscoche, Cristian | |
| dc.contributor.author | Contreras, Carmen | |
| dc.contributor.author | Durand Vara, David Percy | |
| dc.contributor.author | Riveros Ramirez, Maribel Denise | |
| dc.contributor.author | Ruiz, Joaquim | |
| dc.contributor.author | Ochoa Woodell, Theresa Jean | |
| dc.date.accessioned | 2019-02-06T14:45:55Z | |
| dc.date.available | 2019-02-06T14:45:55Z | |
| dc.date.issued | 2016 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12866/5121 | |
| dc.description.abstract | EPEC is an attaching and effacing diarrheal pathogen that carries a large pathogenicity island, locus for enterocyte effacement (LEE). Recently, the pathogenicity island PAI O-122 was described among non-LEE effectors and found to be associated with diarrhea among atypical EPEC strains. It is unknown if incomplete PAI O-122 could be associated with diarrhea duration and severity. To identify these virulence determinants we analyzed 379 EPEC strains isolated from Peruvian children. EPEC was diagnosed by PCR(eae+, stx-) and classified as typical(t-EPEC) or atypical(a-EPEC). To characterize PAI O-122 we amplified three modules by PCR: Module 1(pagC), Module 2(senA, nleB and nleE) and Module 3(lifA/efa-1). To characterize the large ORF lifA/efa-1 we amplified the regions known as efa-N, efa-M and efa-C. Clinical information was obtained from the cohort study. A total of 379 EPEC strains were able to analyze PAI O-122 genes, 128 (10.4%) EPEC strains were isolated from 1235 diarrhea episodes and 251(9.2%) from 2734 healthy controls. t-EPEC strains were isolated from 14.8% (19/128) of children with diarrhea and 25/251(10.0%) from healthy controls. The most frequent PAI O-122 genes were nleE(37.7%), senA(34.6%) and nleB(37.5%), with similar prevalence among diarrhea and control samples. However, lifA/efa-1 was more common among diarrhea cases than healthy control cases (30.5% vs. 21.1%, p<0.05). The presence of complete PAI O-122 was associated with diarrhea episodes of higher severity among single pathogen infection (33.3% vs. 1.8%, p<0.05) mainly due to the presence of a complete lifA/efa-1 gene. In summary, the gene lifA/efa-1 is significantly associated with diarrheal episodes of higher severity, suggesting to be an important virulent factor. | en_US |
| dc.language.iso | eng | |
| dc.publisher | Elsevier | |
| dc.relation.ispartofseries | International Journal of Medical Microbiology | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.subject | Genomic Islands | en_US |
| dc.subject | Animals | en_US |
| dc.subject | Child, Preschool | en_US |
| dc.subject | Children | en_US |
| dc.subject | Diarrhea severity | en_US |
| dc.subject | Diarrhea/microbiology/pathology | en_US |
| dc.subject | DNA, Bacterial/genetics | en_US |
| dc.subject | Enteropathogenic escherichia coli | en_US |
| dc.subject | Enteropathogenic Escherichia coli/genetics/pathogenicity | en_US |
| dc.subject | Escherichia coli Infections/microbiology/pathology | en_US |
| dc.subject | Female | en_US |
| dc.subject | Genes, Bacterial | en_US |
| dc.subject | Humans | en_US |
| dc.subject | Infant | en_US |
| dc.subject | Male | en_US |
| dc.subject | Pathogenicity island O-122 | en_US |
| dc.subject | Peru | en_US |
| dc.subject | Polymerase Chain Reaction | en_US |
| dc.subject | Severity of Illness Index | en_US |
| dc.title | Pathogenicity Island O-122 in enteropathogenic Escherichia coli strains is associated with diarrhea severity in children from Lima Peru | en_US |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | https://doi.org/10.1016/j.ijmm.2016.05.005 | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.06.01 | |
| dc.relation.issn | 1618-0607 |
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