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Validation of the Modification of the Prolonged Papanicolaou Stain for the Diagnosis of Cervical Cancer

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dc.contributor.author Moya-Salazar, Jeel
dc.contributor.author Rojas-Zumaran, Victor
dc.date.accessioned 2019-02-06T14:45:56Z
dc.date.available 2019-02-06T14:45:56Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5125
dc.description.abstract OBJECTIVES: To validate the modification of the prolonged Papanicolaou stain based on the quality control of time, units and staining stages (micro- and macroscopic), and on the stain reactivation based on the control. STUDY DESIGN: A prospective study was performed on 125,358 cervical smears in Lima (Peru). The microscopic quality control included evaluating the cytological characteristics through the staining quality index (SQI) and the quality criteria for samples described by the Bethesda System (TBS). The macroscopic quality control is based on the interslide staining as a set corresponding to a poststaining vertical angle. The stains were reactivated with the addition of 0.15 and 2 g of phosphotungstic acid for Orange G and EA-50, respectively. RESULTS: Both the nonneoplastic and the neoplastic findings comply with the quality criteria of the TBS (SQI value = 0.94). The yield per battery of staining fluctuated between 5,616 and 17,954 Pap smears, and the reactivation of stains produced a stain yield of up to four times the initial amount of the smears, saving USD 5,598.88 from 2013 to 2014. CONCLUSION: The modification of prolonged Papanicolaou staining is an excellent alternative, with a cost-benefit ratio that generates savings and a higher yield per battery of staining. en_US
dc.language.iso eng
dc.publisher Karger
dc.relation.ispartof urn:issn:1938-2650
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Female en_US
dc.subject Humans en_US
dc.subject Papanicolaou Test/methods/standards en_US
dc.subject Phosphotungstic Acid/chemistry en_US
dc.subject Prospective Studies en_US
dc.subject Quality Control en_US
dc.subject Reproducibility of Results en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Staining and Labeling/methods/standards en_US
dc.subject Time Factors en_US
dc.subject Uterine Cervical Neoplasms/diagnosis en_US
dc.subject Vaginal Smears/methods/standards en_US
dc.title Validation of the Modification of the Prolonged Papanicolaou Stain for the Diagnosis of Cervical Cancer en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1159/000444117
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE


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