Validation of the Modification of the Prolonged Papanicolaou Stain for the Diagnosis of Cervical Cancer

Moya-Salazar, Jeel; Rojas-Zumaran, Victor

dc.contributor.author	Moya-Salazar, Jeel
dc.contributor.author	Rojas-Zumaran, Victor
dc.date.accessioned	2019-02-06T14:45:56Z
dc.date.available	2019-02-06T14:45:56Z
dc.date.issued	2016
dc.identifier.uri	https://hdl.handle.net/20.500.12866/5125
dc.description.abstract	OBJECTIVES: To validate the modification of the prolonged Papanicolaou stain based on the quality control of time, units and staining stages (micro- and macroscopic), and on the stain reactivation based on the control. STUDY DESIGN: A prospective study was performed on 125,358 cervical smears in Lima (Peru). The microscopic quality control included evaluating the cytological characteristics through the staining quality index (SQI) and the quality criteria for samples described by the Bethesda System (TBS). The macroscopic quality control is based on the interslide staining as a set corresponding to a poststaining vertical angle. The stains were reactivated with the addition of 0.15 and 2 g of phosphotungstic acid for Orange G and EA-50, respectively. RESULTS: Both the nonneoplastic and the neoplastic findings comply with the quality criteria of the TBS (SQI value = 0.94). The yield per battery of staining fluctuated between 5,616 and 17,954 Pap smears, and the reactivation of stains produced a stain yield of up to four times the initial amount of the smears, saving USD 5,598.88 from 2013 to 2014. CONCLUSION: The modification of prolonged Papanicolaou staining is an excellent alternative, with a cost-benefit ratio that generates savings and a higher yield per battery of staining.	en_US
dc.language.iso	eng
dc.publisher	Karger
dc.relation.ispartofseries	Acta Cytologica
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Female	en_US
dc.subject	Humans	en_US
dc.subject	Papanicolaou Test/methods/standards	en_US
dc.subject	Phosphotungstic Acid/chemistry	en_US
dc.subject	Prospective Studies	en_US
dc.subject	Quality Control	en_US
dc.subject	Reproducibility of Results	en_US
dc.subject	Sensitivity and Specificity	en_US
dc.subject	Staining and Labeling/methods/standards	en_US
dc.subject	Time Factors	en_US
dc.subject	Uterine Cervical Neoplasms/diagnosis	en_US
dc.subject	Vaginal Smears/methods/standards	en_US
dc.title	Validation of the Modification of the Prolonged Papanicolaou Stain for the Diagnosis of Cervical Cancer	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1159/000444117
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.02.03
dc.relation.issn	1938-2650