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Cross-Reactivity Pattern of Asian and American Human Gnathostomiasis in Western Blot Assays Using Crude Antigens Prepared from Gnathostoma spinigerum and Gnathostoma binucleatum Third-Stage Larvae

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dc.contributor.author Neumayr, Andreas
dc.contributor.author Ollague, Jose
dc.contributor.author Bravo, Francisco
dc.contributor.author Gotuzzo, Eduardo
dc.contributor.author Jimenez, Pedro
dc.contributor.author Norton, Scott-A.
dc.contributor.author Doanh, Pham-Ngoc
dc.contributor.author Nawa, Yukifumi
dc.contributor.author Horii, Yoichiro
dc.contributor.author Nickel, Beatrice
dc.contributor.author Marti, Hanspeter
dc.date.accessioned 2019-02-06T14:45:56Z
dc.date.available 2019-02-06T14:45:56Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5127
dc.description.abstract Gnathostomiasis is a zoonotic parasitosis endemic in many Asian and some Latin American countries. Most human infections are caused by Gnathostoma spinigerum in Asia and Gnathostoma binucleatum in the Americas, and recently, imported cases have been increasing among travelers returning from endemic regions. Confirmation of the clinical diagnosis relies largely on serologic tests, with a G. spinigerum-antigen-based immunoblot currently being the diagnostic method of choice. However, we repeatedly experienced that sera from patients with clinically suspected American gnathostomiasis gave negative results in this assay. Therefore, we used homologous methods to prepare G. spinigerum- and G. binucleatum-antigen-based immunoblot assays, and evaluated the cross-reactivity of the two assays. The results show incomplete cross-reactivity between the two assays: the G. spinigerum-antigen-based immunoblot apparently only detects Asian gnathostomiasis caused by G. spinigerum, whereas the G. binucleatum-antigen-based immunoblot is apparently capable of detecting American as well as Asian gnathostomiasis. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartof urn:issn:1476-1645
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject Antibodies, Helminth/biosynthesis/chemistry en_US
dc.subject Antigens, Helminth/immunology/isolation & purification en_US
dc.subject Asia en_US
dc.subject Blotting, Western/methods en_US
dc.subject Cross Reactions en_US
dc.subject Eels/parasitology en_US
dc.subject Gnathostoma/anatomy & histology/classification/immunology/isolation & purification en_US
dc.subject Gnathostomiasis/diagnosis/parasitology en_US
dc.subject Humans en_US
dc.subject Larva/anatomy & histology/chemistry/classification/immunology en_US
dc.subject United States en_US
dc.title Cross-Reactivity Pattern of Asian and American Human Gnathostomiasis in Western Blot Assays Using Crude Antigens Prepared from Gnathostoma spinigerum and Gnathostoma binucleatum Third-Stage Larvae en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.16-0176
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06


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