Universidad Peruana Cayetano Heredia

Anti-Taenia solium monoclonal antibodies for the detection of parasite antigens in body fluids from patients with neurocysticercosis

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dc.contributor.author Paredes, Adriana
dc.contributor.author Saenz, Patricia
dc.contributor.author Marzal, Miguel-W.
dc.contributor.author Orrego Solano, Miguel Ángel
dc.contributor.author Castillo Berrios, Yesenia
dc.contributor.author Rivera, Andrea
dc.contributor.author Mahanty, Siddhartha
dc.contributor.author Guerra-Giraldez, Cristina
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Nash, Theodore-E.
dc.date.accessioned 2019-02-06T14:45:57Z
dc.date.available 2019-02-06T14:45:57Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5135
dc.description.abstract Neurocysticercosis (NCC), an infection of the brain by Taenia solium (Ts) cysts, is the most common cause of adult-onset epilepsy in developing countries. Serological testing consists primarily of varying methods to detect antibodies in body fluids and more recently antigen (Ag) detection assays to identify individuals or animals with viable parasites. Antigen assays currently in use employ monoclonal antibodies (mAbs) raised against T. saginata, which have known cross reactivity to animal cestodes but are highly specific in human samples. We produced, characterized and tested 21 mAbs raised against T. solium whole cyst antigens, vesicular fluid or excretory secretory products. Reactivity of the TsmAbs against specific cyst structures was determined using immunofluorescence and immunohistochemistry on histological sections of Ts muscle cysts. Four TsmAbs reacted to vesicular space alone, 9 to the neck and cyst wall, one to the neck and vesicular space and 7 to the neck, cyst wall and vesicular space. An in-house ELISA assay to detect circulating Ts antigen, using the TsmAbs as capture antibodies and a rabbit polyclonal anti-Ts whole cyst antibody as a detector antibody demonstrated that eight of the 21 TsmAbs detected antigens in known en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Experimental Parasitology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject Antibodies, Helminth/immunology en_US
dc.subject Antibodies, Monoclonal/immunology/metabolism en_US
dc.subject Antibody Specificity en_US
dc.subject Antigens, Helminth/analysis/blood/urine en_US
dc.subject Bile/immunology en_US
dc.subject Capture ELISA en_US
dc.subject Diagnosis en_US
dc.subject Enzyme-Linked Immunosorbent Assay en_US
dc.subject Female en_US
dc.subject Fluorescent Antibody Technique en_US
dc.subject Hybridomas/immunology en_US
dc.subject Mice en_US
dc.subject Mice, Inbred BALB C en_US
dc.subject Monoclonal antibodies en_US
dc.subject Neurocysticercosis en_US
dc.subject Neurocysticercosis/diagnosis/immunology en_US
dc.subject Rabbits en_US
dc.subject Species Specificity en_US
dc.subject Swine en_US
dc.subject Taenia solium en_US
dc.subject Taenia solium/immunology en_US
dc.title Anti-Taenia solium monoclonal antibodies for the detection of parasite antigens in body fluids from patients with neurocysticercosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.exppara.2016.03.025
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn 1090-2449


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