Universidad Peruana Cayetano Heredia

Recombination in pe/ppe genes contributes to genetic variation in Mycobacterium tuberculosis lineages

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dc.contributor.author Phelan, Jody-E.
dc.contributor.author Coll, Francesc
dc.contributor.author Bergval, Indra
dc.contributor.author Anthony, Richard-M.
dc.contributor.author Warren, Rob
dc.contributor.author Sampson, Samantha-L.
dc.contributor.author Gey-van-Pittius, Nicolaas-C.
dc.contributor.author Glynn, Judith-R.
dc.contributor.author Crampin, Amelia-C.
dc.contributor.author Alves, Adriana
dc.contributor.author Bessa, Theolis-Barbosa
dc.contributor.author Campino, Susana
dc.contributor.author Dheda, Keertan
dc.contributor.author Grandjean, Louis
dc.contributor.author Hasan, Rumina
dc.contributor.author Hasan, Zahra
dc.contributor.author Miranda, Anabela
dc.contributor.author Moore, David Alexander James
dc.contributor.author Panaiotov, Stefan
dc.contributor.author Perdigao, Joao
dc.contributor.author Portugal, Isabel
dc.contributor.author Sheen Cortavarria, Patricia
dc.contributor.author de-Oliveira-Sousa, Erivelton
dc.contributor.author Streicher, Elizabeth-M.
dc.contributor.author van-Helden, Paul-D.
dc.contributor.author Viveiros, Miguel
dc.contributor.author Hibberd, Martin-L.
dc.contributor.author Pain, Arnab
dc.contributor.author McNerney, Ruth
dc.contributor.author Clark, Taane-G.
dc.date.accessioned 2019-02-06T14:45:57Z
dc.date.available 2019-02-06T14:45:57Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5143
dc.description.abstract BACKGROUND: Approximately 10% of the Mycobacterium tuberculosis genome is made up of two families of genes that are poorly characterized due to their high GC content and highly repetitive nature. The PE and PPE families are typified by their highly conserved N-terminal domains that incorporate proline-glutamate (PE) and proline-proline-glutamate (PPE) signature motifs. They are hypothesised to be important virulence factors involved with host-pathogen interactions, but their high genetic variability and complexity of analysis means they are typically disregarded in genome studies. RESULTS: To elucidate the structure of these genes, 518 genomes from a diverse international collection of clinical isolates were de novo assembled. A further 21 reference M. tuberculosis complex genomes and long read sequence data were used to validate the approach. SNP analysis revealed that variation in the majority of the 168 pe/ppe genes studied was consistent with lineage. Several recombination hotspots were identified, notably pe_pgrs3 and pe_pgrs17. Evidence of positive selection was revealed in 65 pe/ppe genes, including epitopes potentially binding to major histocompatibility complex molecules. CONCLUSIONS: This, the first comprehensive study of the pe and ppe genes, provides important insight into M. tuberculosis diversity and has significant implications for vaccine development. en_US
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartofseries BMC Genomics
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Genes, Bacterial en_US
dc.subject Multigene Family en_US
dc.subject Polymorphism, Single Nucleotide en_US
dc.subject Recombination, Genetic en_US
dc.subject DNA, Bacterial/genetics en_US
dc.subject Evolution, Molecular en_US
dc.subject Genome, Bacterial en_US
dc.subject Genomics/methods en_US
dc.subject Genotype en_US
dc.subject Mutation en_US
dc.subject Mycobacterium tuberculosis/genetics en_US
dc.subject Phylogeny en_US
dc.subject Selection, Genetic en_US
dc.subject Sequence Analysis, DNA en_US
dc.title Recombination in pe/ppe genes contributes to genetic variation in Mycobacterium tuberculosis lineages en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1186/s12864-016-2467-y
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.07
dc.relation.issn 1471-2164


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