Universidad Peruana Cayetano Heredia

An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp Infections

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dc.contributor.author Saldarriaga, Omar-A.
dc.contributor.author Castellanos-Gonzalez, Alejandro
dc.contributor.author Porrozzi, Renato
dc.contributor.author Baldeviano, Gerald-C.
dc.contributor.author Lescano Guevara, Andres Guillermo
dc.contributor.author de-Los-Santos, Maxy-B.
dc.contributor.author Fernandez, Olga-L.
dc.contributor.author Saravia, Nancy-G.
dc.contributor.author Costa, Erika
dc.contributor.author Melby, Peter-C.
dc.contributor.author Travi, Bruno-L.
dc.date.accessioned 2019-02-06T14:45:58Z
dc.date.available 2019-02-06T14:45:58Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5155
dc.description.abstract Cutaneous and mucosal leishmaniasis is widely distributed in Central and South America. Leishmania of the Viannia subgenus are the most frequent species infecting humans. L. (V.) braziliensis, L. (V.) panamensis are also responsible for metastatic mucosal leishmaniasis. Conventional or real time PCR is a more sensitive diagnostic test than microscopy, but the cost and requirement for infrastructure and trained personnel makes it impractical in most endemic regions. Primary health systems need a sensitive and specific point of care (POC) diagnostic tool. We developed a novel POC molecular diagnostic test for cutaneous leishmaniasis caused by Leishmania (Viannia) spp. Parasite DNA was amplified using isothermal Recombinase Polymerase Amplification (RPA) with primers and probes that targeted the kinetoplast DNA. The amplification product was detected by naked eye with a lateral flow (LF) immunochromatographic strip. The RPA-LF had an analytical sensitivity equivalent to 0.1 parasites per reaction. The test amplified the principal L. Viannia species from multiple countries: L. (V.) braziliensis (n = 33), L. (V.) guyanensis (n = 17), L. (V.) panamensis (n = 9). The less common L. (V.) lainsoni, L. (V.) shawi, and L. (V.) naiffi were also amplified. No amplification was observed in parasites of the L. (Leishmania) subgenus. In a small number of clinical samples (n = 13) we found 100% agreement between PCR and RPA-LF. The high analytical sensitivity and clinical validation indicate the test could improve the efficiency of diagnosis, especially in chronic lesions with submicroscopic parasite burdens. Field implementation of the RPA-LF test could contribute to management and control of cutaneous and mucosal leishmaniasis. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Point-of-Care Systems en_US
dc.subject Chromatography, Affinity en_US
dc.subject DNA Primers/genetics en_US
dc.subject DNA, Kinetoplast/genetics en_US
dc.subject DNA, Protozoan/genetics en_US
dc.subject Humans en_US
dc.subject Leishmania/genetics/isolation & purification en_US
dc.subject Leishmaniasis, Cutaneous/diagnosis en_US
dc.subject Molecular Diagnostic Techniques/methods en_US
dc.subject Nucleic Acid Amplification Techniques en_US
dc.subject Oligonucleotide Probes/genetics en_US
dc.subject Sensitivity and Specificity en_US
dc.title An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp Infections en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0004638
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


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