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Occurrence of Giardia intestinalis and Cryptosporidium sp in wastewater samples from Sao Paulo State, Brazil, and Lima, Peru

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dc.contributor.author Ulloa-Stanojlovic, Francisco-Miroslav
dc.contributor.author Aguiar, Bruna
dc.contributor.author Jara Salazar, Luis Miguel
dc.contributor.author Zanoli Sato, Maria Ines
dc.contributor.author Arzola Guerrero, Juana
dc.contributor.author Hachich, Elayse
dc.contributor.author Rogerio Matte, Glavur
dc.contributor.author Dropa, Milena
dc.contributor.author Matte, Maria Helena
dc.contributor.author Silva de Araujo, Ronalda
dc.date.accessioned 2019-02-06T14:48:07Z
dc.date.available 2019-02-06T14:48:07Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/20.500.12866/5172
dc.description.abstract The objectives of the study were to detect and genotype Cryptosporidium spp. and Giardia intestinalis in wastewater samples obtained from five cities with high transit of people in the State of Sao Paulo, Brazil, and at the entrance of a Wastewater Treatment Plant (WWTP) in Lima, Peru. Samples were collected and concentrated by centrifugation. The genomic DNA was extracted for molecular characterization by nested PCR for Cryptosporidium and double nested PCR for Giardia, followed by sequencing and phylogenetic analysis. G. intestinalis was found in 63.6 % of the samples, and the human assemblages A and B were identified. Cryptosporidium sp. was found in 36.4 % of the samples, and the species were corresponding to Cryptosporidium hominis, Cryptosporidium cuniculus, and Cryptosporidium muris. Results revealed the presence of human pathogenic Cryptosporidium species and G. intestinalis human pathogenic assemblages. Molecular tools highlight the importance to map the genetic diversity of these parasites, as well as to detect their epidemiological circulation pathway in the environment. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartofseries Environmental Science and Pollution Research
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Cryptosporidium sp. en_US
dc.subject Giardia intestinalis en_US
dc.subject PCR en_US
dc.subject Public health en_US
dc.subject Wastewater en_US
dc.subject Brazil en_US
dc.subject Cities en_US
dc.subject Cryptosporidium/genetics/isolation & purification en_US
dc.subject Genotype en_US
dc.subject Giardia lamblia/genetics/isolation & purification en_US
dc.subject Peru en_US
dc.subject Phylogeny en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject RNA, Protozoan/genetics en_US
dc.subject RNA, Ribosomal, 18S/genetics en_US
dc.subject Sequence Analysis, DNA en_US
dc.subject Waste Water/parasitology en_US
dc.title Occurrence of Giardia intestinalis and Cryptosporidium sp in wastewater samples from Sao Paulo State, Brazil, and Lima, Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s11356-016-7537-9
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.05.08
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.05
dc.relation.issn 1614-7499


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