Mostrar el registro sencillo del ítem
dc.contributor.author | Ulloa-Stanojlovic, Francisco-Miroslav | |
dc.contributor.author | Aguiar, Bruna | |
dc.contributor.author | Jara Salazar, Luis Miguel | |
dc.contributor.author | Zanoli Sato, Maria Ines | |
dc.contributor.author | Arzola Guerrero, Juana | |
dc.contributor.author | Hachich, Elayse | |
dc.contributor.author | Rogerio Matte, Glavur | |
dc.contributor.author | Dropa, Milena | |
dc.contributor.author | Matte, Maria Helena | |
dc.contributor.author | Silva de Araujo, Ronalda | |
dc.date.accessioned | 2019-02-06T14:48:07Z | |
dc.date.available | 2019-02-06T14:48:07Z | |
dc.date.issued | 2016 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12866/5172 | |
dc.description.abstract | The objectives of the study were to detect and genotype Cryptosporidium spp. and Giardia intestinalis in wastewater samples obtained from five cities with high transit of people in the State of Sao Paulo, Brazil, and at the entrance of a Wastewater Treatment Plant (WWTP) in Lima, Peru. Samples were collected and concentrated by centrifugation. The genomic DNA was extracted for molecular characterization by nested PCR for Cryptosporidium and double nested PCR for Giardia, followed by sequencing and phylogenetic analysis. G. intestinalis was found in 63.6 % of the samples, and the human assemblages A and B were identified. Cryptosporidium sp. was found in 36.4 % of the samples, and the species were corresponding to Cryptosporidium hominis, Cryptosporidium cuniculus, and Cryptosporidium muris. Results revealed the presence of human pathogenic Cryptosporidium species and G. intestinalis human pathogenic assemblages. Molecular tools highlight the importance to map the genetic diversity of these parasites, as well as to detect their epidemiological circulation pathway in the environment. | en_US |
dc.language.iso | eng | |
dc.publisher | Springer | |
dc.relation.ispartofseries | Environmental Science and Pollution Research | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
dc.subject | Cryptosporidium sp. | en_US |
dc.subject | Giardia intestinalis | en_US |
dc.subject | PCR | en_US |
dc.subject | Public health | en_US |
dc.subject | Wastewater | en_US |
dc.subject | Brazil | en_US |
dc.subject | Cities | en_US |
dc.subject | Cryptosporidium/genetics/isolation & purification | en_US |
dc.subject | Genotype | en_US |
dc.subject | Giardia lamblia/genetics/isolation & purification | en_US |
dc.subject | Peru | en_US |
dc.subject | Phylogeny | en_US |
dc.subject | Polymerase Chain Reaction | en_US |
dc.subject | RNA, Protozoan/genetics | en_US |
dc.subject | RNA, Ribosomal, 18S/genetics | en_US |
dc.subject | Sequence Analysis, DNA | en_US |
dc.subject | Waste Water/parasitology | en_US |
dc.title | Occurrence of Giardia intestinalis and Cryptosporidium sp in wastewater samples from Sao Paulo State, Brazil, and Lima, Peru | en_US |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | https://doi.org/10.1007/s11356-016-7537-9 | |
dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.05.08 | |
dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.03.05 | |
dc.relation.issn | 1614-7499 |
Ficheros | Tamaño | Formato | Ver |
---|---|---|---|
No hay ficheros asociados a este ítem. |