Domestic Pig (Sus scrofa) as an Animal Model for Experimental Trypanosoma cruzi Infection

Gavidia, Cesar M.; Malaga, Edith; Gilman, Robert Hugh; Yauri, Veronica; Bern, Caryn; Cabello, Ines; Verastegui Pimentel, Manuela Renee; Castro-Sesquen, Yagahira E.; Recuenco, Fernando; Angulo, Noelia

dc.contributor.author	Yauri, Veronica
dc.contributor.author	Castro-Sesquen, Yagahira E.
dc.contributor.author	Verastegui Pimentel, Manuela Renee
dc.contributor.author	Angulo, Noelia
dc.contributor.author	Recuenco, Fernando
dc.contributor.author	Cabello, Ines
dc.contributor.author	Malaga, Edith
dc.contributor.author	Bern, Caryn
dc.contributor.author	Gavidia, Cesar M.
dc.contributor.author	Gilman, Robert Hugh
dc.date.accessioned	2019-02-06T14:48:08Z
dc.date.available	2019-02-06T14:48:08Z
dc.date.issued	2016
dc.identifier.uri	https://hdl.handle.net/20.500.12866/5179
dc.description.abstract	Pigs were infected with a Bolivian strain of Trypanosoma cruzi (genotype I) and evaluated up to 150 days postinoculation (dpi) to determine the use of pigs as an animal model of Chagas disease. Parasitemia was observed in the infected pigs during the acute phase (15-40 dpi). Anti-T. cruzi immunoglobulin M was detected during 15-75 dpi; high levels of anti-T. cruzi immunoglobulin G were detected in all infected pigs from 75 to 150 dpi. Parasitic DNA was observed by western blot (58%, 28/48) and polymerase chain reaction (27%, 13/48) in urine samples, and in the brain (75%, 3/4), spleen (50%, 2/4), and duodenum (25%, 1/4), but no parasitic DNA was found in the heart, colon, and kidney. Parasites were not observed microscopically in tissues samples, but mild inflammation, vasculitis, and congestion was observed in heart, brain, kidney, and spleen. This pig model was useful for the standardization of the urine test because of the higher volume that can be obtained as compared with other small animal models. However, further experiments are required to observe pathological changes characteristic of Chagas disease in humans.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Disease Models, Animal	en_US
dc.subject	Animals	en_US
dc.subject	Antibodies, Protozoan/blood	en_US
dc.subject	Blotting, Western	en_US
dc.subject	Chagas Disease/parasitology	en_US
dc.subject	DNA, Protozoan/isolation & purification/urine	en_US
dc.subject	Female	en_US
dc.subject	Immunoglobulin G/blood	en_US
dc.subject	Parasitemia	en_US
dc.subject	Swine	en_US
dc.subject	Time Factors	en_US
dc.subject	Tissue Distribution	en_US
dc.subject	Trypanosoma cruzi/physiology	en_US
dc.title	Domestic Pig (Sus scrofa) as an Animal Model for Experimental Trypanosoma cruzi Infection	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.15-0233
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645