DSpace Repository

Development and analysis of furazolidone-resistant Escherichia coli mutants

Show simple item record

dc.contributor.author Martinez-Puchol, Sandra
dc.contributor.author Gomes, Claudia
dc.contributor.author Pons, Maria J.
dc.contributor.author Ruiz-Roldan, Lidia
dc.contributor.author Torrents de la Pena, Alba
dc.contributor.author Ochoa, Theresa J.
dc.contributor.author Ruiz, Joaquim
dc.date.accessioned 2019-02-06T14:52:12Z
dc.date.available 2019-02-06T14:52:12Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/5270
dc.description.abstract Furazolidone-resistant mutants were obtained from four clinical isolates of diarrhoeagenic Escherichia coli. The stability of the resistance and the frequency of mutation were established. The minimal inhibitory concentration of furazolidone, nitrofurantoin, nalidixic acid, ampicillin, chloramphenicol and tetracycline was established both in the presence and absence of the efflux pump inhibitor Phe-Arg-beta-Naphtylamyde. The presence of mutations in the nitroreductase genes nfsA and nfsB was analysed by PCR; sequencing and their enzymatic activity was assessed by a spectrophotometric assay. Alterations in outer membrane proteins were studied by SDS-PAGE. The frequency of mutation ranged from <9.6 x 10(-10) to 9.59 x 10(-7) . Neither an effect on efflux pumps inhibited by Phe-Arg-beta-Naphtylamyde nor cross-resistance with the antibiotics studied was observed. Nineteen mutants (52.94%) presented mutations in the nitroreductase-encoding genes: 17 in the nfsA gene (15 mutants with an internal stop codon, 2 with amino acid changes), 2 in the nfsB (all amino acid changes). Alterations in the outer membrane proteins OmpA and OmpW were also observed. Although more studies are necessary to find other resistance mechanisms, present data showed the low potential of selecting furazolidone-resistant mutants, together with the lack of cross-resistance with unrelated antimicrobial agents. en_US
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof urn:issn:1600-0463
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Mutation en_US
dc.subject Microbial Sensitivity Tests en_US
dc.subject Anti-Bacterial Agents/pharmacology en_US
dc.subject Electrophoresis, Polyacrylamide Gel en_US
dc.subject Bacterial Outer Membrane Proteins/genetics en_US
dc.subject Dipeptides en_US
dc.subject Drug Resistance, Multiple, Bacterial/genetics en_US
dc.subject Enterobacteriaceae en_US
dc.subject Escherichia coli Proteins/genetics en_US
dc.subject Escherichia coli/drug effects/genetics en_US
dc.subject Furazolidone/pharmacology en_US
dc.subject Nitrofuran en_US
dc.subject Nitroreductases/genetics en_US
dc.subject resistance mechanisms en_US
dc.title Development and analysis of furazolidone-resistant Escherichia coli mutants en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1111/apm.12401
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics