Universidad Peruana Cayetano Heredia

Design of a predicted MHC restricted short peptide immunodiagnostic and vaccine candidate for Fowl adenovirus C in chicken infection

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dc.contributor.author Valdivia-Olarte, Hugo
dc.contributor.author Requena, David
dc.contributor.author Ramirez, Manuel
dc.contributor.author Saravia, Luis E.
dc.contributor.author Izquierdo, Ray
dc.contributor.author Falconi-Agapito, Francesca
dc.contributor.author Zavaleta, Milagros
dc.contributor.author Best, Ivan
dc.contributor.author Fernandez-Diaz, Manolo
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.date.accessioned 2019-02-06T14:52:14Z
dc.date.available 2019-02-06T14:52:14Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/5276
dc.description.abstract Fowl adenoviruses (FAdVs) are the ethiologic agents of multiple pathologies in chicken. There are five different species of FAdVs grouped as FAdV-A, FAdV-B, FAdV-C, FAdV-D, and FAdV-E. It is of interest to develop immunodiagnostics and vaccine candidate for Peruvian FAdV-C in chicken infection using MHC restricted short peptide candidates. We sequenced the complete genome of one FAdV strain isolated from a chicken of a local farm. A total of 44 protein coding genes were identified in each genome. We sequenced twelve Cobb chicken MHC alleles from animals of different farms in the central coast of Peru, and subsequently determined three optimal human MHC-I and four optimal human MHC-II substitute alleles for MHC-peptide prediction. The potential MHC restricted short peptide epitope-like candidates were predicted using human specific (with determined suitable chicken substitutes) NetMHC MHC-peptide prediction model with web server features from all the FAdV genomes available. FAdV specific peptides with calculated binding values to known substituted chicken MHC-I and MHC-II were further filtered for diagnostics and potential vaccine epitopes. Promiscuity to the 3/4 optimal human MHC-I/II alleles and conservation among the available FAdV genomes was considered in this analysis. The localization on the surface of the protein was considered for class II predicted peptides. Thus, a set of class I and class II specific peptides from FAdV were reported in this study. Hence, a multiepitopic protein was built with these peptides, and subsequently tested to confirm the production of specific antibodies in chicken. en_US
dc.language.iso eng
dc.publisher Biomedical Informatics
dc.relation.ispartofseries Bioinformation
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject MHC restricted peptide en_US
dc.subject immunodiagnostic en_US
dc.subject Vaccine candidate en_US
dc.subject Fowl adenovirus C en_US
dc.subject Chicken infection en_US
dc.title Design of a predicted MHC restricted short peptide immunodiagnostic and vaccine candidate for Fowl adenovirus C in chicken infection en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.6026/97320630011460
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.02.03
dc.relation.issn 0973-2063


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