DSpace Repository

Virulence factors and mechanisms of antimicrobial resistance in Shigella strains from periurban areas of Lima (Peru)

Show simple item record

dc.contributor.author Lluque, Angela
dc.contributor.author Mosquito, Susan
dc.contributor.author Gomes, Claudia
dc.contributor.author Riveros Ramirez, Maribel Denise
dc.contributor.author Durand Vara, David Percy
dc.contributor.author Tilley, Drake H.
dc.contributor.author Bernal, Maria
dc.contributor.author Prada, Ana
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.contributor.author Ruiz, Joaquim
dc.date.accessioned 2019-02-06T14:52:37Z
dc.date.available 2019-02-06T14:52:37Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/5330
dc.description.abstract The study was aimed to describe the serotype, mechanisms of antimicrobial resistance, and virulence determinants in Shigella spp. isolated from Peruvian children. Eighty three Shigella spp. were serogrouped and serotyped being established the antibiotic susceptibility. The presence of 12 virulence factors (VF) and integrase 1 and 2, along with commonly found antibiotic resistance genes was established by PCR. S. flexneri was the most relevant serogroup (55 isolates, 66%), with serotype 2a most frequently detected (27 of 55, 49%), followed by S. boydii and S. sonnei at 12 isolates each (14%) and S. dysenteriae (four isolates, 5%). Fifty isolates (60%) were multi-drug resistant (MDR) including 100% of S. sonnei and 64% of S. flexneri. Resistance levels were high to trimethoprim-sulfamethoxazole (86%), tetracycline (74%), ampicillin (67%), and chloramphenicol (65%). Six isolates showed decreased azithromycin susceptibility. No isolate was resistant to nalidixic acid, ciprofloxacin, nitrofurantoin, or ceftriaxone. The most frequent resistance genes were sul2 (95%), tet(B) (92%), cat (80%), dfrA1 (47%), blaOXA-1like (40%), with intl1 and intl2 detected in 51 and 52% of the isolates, respectively. Thirty-one different VF profiles were observed, being the ipaH (100%), sen (77%), virA and icsA (75%) genes the most frequently found. Differences in the prevalence of VF were observed between species with S. flexneri isolates, particularly serotype 2a, possessing high numbers of VF. In conclusion, this study highlights the high heterogeneity of Shigella VF and resistance genes, and prevalence of MDR organisms within this geographic region. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries International Journal of Medical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Infant en_US
dc.subject Peru/epidemiology en_US
dc.subject Antimicrobial resistance en_US
dc.subject Drug Resistance, Bacterial en_US
dc.subject Anti-Bacterial Agents/pharmacology en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject Microbial Sensitivity Tests en_US
dc.subject Suburban Population en_US
dc.subject Autotransporters en_US
dc.subject Dysentery, Bacillary/epidemiology/microbiology en_US
dc.subject Enterotoxins en_US
dc.subject Ipa en_US
dc.subject Serogroup en_US
dc.subject Shigella serotypes en_US
dc.subject Shigella/classification/drug effects/isolation & purification/pathogenicity en_US
dc.subject Shigellosis en_US
dc.subject Virulence Factors/genetics en_US
dc.title Virulence factors and mechanisms of antimicrobial resistance in Shigella strains from periurban areas of Lima (Peru) en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.ijmm.2015.04.005
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1618-0607


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics