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Evaluation of a New Immunochromatographic Test Using Recombinant Antigen B8/1 for Diagnosis of Cystic Echinococcosis

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dc.contributor.author Santivanez, Saul J.
dc.contributor.author Rodriguez, Mary L.
dc.contributor.author Rodriguez, Silvia
dc.contributor.author Sako, Yashuito
dc.contributor.author Nkouawa, Agathe
dc.contributor.author Kobayashi, Yukuharu
dc.contributor.author Sotomayor, Alfredo L.
dc.contributor.author Peralta, Julio E.
dc.contributor.author Valcarcel, Maria
dc.contributor.author Gonzalez, Armando E.
dc.contributor.author Garcia, Hector H.
dc.contributor.author Ito, Akira
dc.date.accessioned 2019-02-06T14:52:38Z
dc.date.available 2019-02-06T14:52:38Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/5342
dc.description.abstract Diagnosis of cystic echinococcosis (CE) is based on the identification of the cyst(s) by imaging, using immunodiagnostic tests mainly as complementary tools in clinical settings. Among the antigens used for immunodiagnosis, previous studies described a good performance of the recombinant antigen B8/1 (rAgB) in an enzyme-linked immunosorbent assay (ELISA) format; however, in remote parts of areas where the disease is endemic, the implementation of an ELISA is difficult, so a more simple, rapid, and reliable method such as the immunochromatographic test (ICT) is required. In this study, using a set of 50 serum samples from patients with surgically confirmed CE, we compared the performance of an ICT and that of an ELISA using the rAgB. The overall sensitivities of ICT and ELISA were not statistically different (78% versus 72%; P = 0.36). The overall agreement between both tests was moderate (kappa = 0.41; P < 0.01). Concordance between ICT and ELISA was substantial or almost perfect for patients with liver involvement (kappa = 0.65; P < 0.001) and patients with more than one hydatid cyst (kappa = 0.82; P < 0.001), respectively. Moreover, specificity analysis using a total of 88 serum samples from healthy individuals (n = 20) and patients (n = 68) with other parasitic infections revealed that ICT had a specificity of 89.8%. ICT and ELISA had similar performance for the detection of specific antibodies to E. granulosus, and ICT had a high specificity, opening the possibility of using ICT as a screening tool in rural settings. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartof urn:issn:1098-660X
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Enzyme-Linked Immunosorbent Assay en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Antibodies, Helminth/blood en_US
dc.subject Antigens, Helminth/immunology en_US
dc.subject Chromatography, Affinity/methods en_US
dc.subject Echinococcosis/diagnosis en_US
dc.subject Recombinant Proteins/immunology en_US
dc.title Evaluation of a New Immunochromatographic Test Using Recombinant Antigen B8/1 for Diagnosis of Cystic Echinococcosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/JCM.02157-15
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01

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