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Comparison of Hybribio GenoArray and Roche human papillomavirus (HPV) linear array for HPV genotyping in anal swab samples

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dc.contributor.author Low, Huey Chi
dc.contributor.author Silver, Michelle I.
dc.contributor.author Brown, Brandon J.
dc.contributor.author Leng, Chan Yoon
dc.contributor.author Blas Blas, Magaly Marlitz
dc.contributor.author Gravitt, Patti E.
dc.contributor.author Woo, Yin Ling
dc.date.accessioned 2019-02-06T14:52:38Z
dc.date.available 2019-02-06T14:52:38Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/5343
dc.description.abstract Human papillomavirus (HPV) is causally associated with anal cancer, as HPV DNA is detected in up to 90% of anal intraepithelial neoplasias and anal cancers. With the gradual increase of anal cancer rates, there is a growing need to establish reliable and clinically relevant methods to detect anal cancer precursors. In resource-limited settings, HPV DNA detection is a potentially relevant tool for anal cancer screening. Here, we evaluated the performance of the Hybribio GenoArray (GA) for genotyping HPV in anal samples, against the reference standard Roche Linear Array (LA). Anal swab samples were obtained from sexually active men who have sex with men. Following DNA extraction, each sample was genotyped using GA and LA. The overall interassay agreement, type-specific, and single and multiple genotype agreements were evaluated by kappa statistics and McNemar's chi(2) tests. Using GA and LA, 68% and 76% of samples were HPV DNA positive, respectively. There was substantial interassay agreements for the detection of all HPV genotypes (kappa = 0.70, 86% agreement). Although LA was able to detect more genotypes per sample, the interassay agreement was acceptable (kappa = 0.53, 63% agreement). GA had poorer specific detection of HPV genotypes 35, 42, and 51 (kappa < 0.60). In conclusion, GA and LA showed good interassay agreement for the detection of most HPV genotypes in anal samples. However, the detection of HPV DNA in up to 76% of anal samples warrants further evaluation of its clinical significance. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Journal of Clinical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Male en_US
dc.subject Homosexuality, Male en_US
dc.subject Anal Canal/virology en_US
dc.subject Anus Neoplasms/diagnosis/virology en_US
dc.subject Early Detection of Cancer/methods en_US
dc.subject Genotyping Techniques/methods en_US
dc.subject Papillomaviridae/classification/genetics/isolation & purification en_US
dc.subject Papillomavirus Infections/complications/virology en_US
dc.title Comparison of Hybribio GenoArray and Roche human papillomavirus (HPV) linear array for HPV genotyping in anal swab samples en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/JCM.02274-14
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1098-660X


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