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Identification and Characterization of Microsatellite Markers Derived from the Whole Genome Analysis of Taenia solium

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dc.contributor.author Pajuelo, Monica J.
dc.contributor.author Eguiluz, Maria
dc.contributor.author Dahlstrom, Eric
dc.contributor.author Requena, David
dc.contributor.author Guzman, Frank
dc.contributor.author Ramirez, Manuel
dc.contributor.author Sheen, Patricia
dc.contributor.author Frace, Michael
dc.contributor.author Sammons, Scott
dc.contributor.author Cama, Vitaliano
dc.contributor.author Anzick, Sarah
dc.contributor.author Bruno, Dan
dc.contributor.author Mahanty, Siddhartha
dc.contributor.author Wilkins, Patricia
dc.contributor.author Nash, Theodore
dc.contributor.author Gonzalez, Armando
dc.contributor.author Garcia, Hector H.
dc.contributor.author Gilman, Robert H.
dc.contributor.author Porcella, Steve
dc.contributor.author Zimic, Mirko
dc.date.accessioned 2019-02-06T14:53:08Z
dc.date.available 2019-02-06T14:53:08Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/5381
dc.description.abstract BACKGROUND: Infections with Taenia solium are the most common cause of adult acquired seizures worldwide, and are the leading cause of epilepsy in developing countries. A better understanding of the genetic diversity of T. solium will improve parasite diagnostics and transmission pathways in endemic areas thereby facilitating the design of future control measures and interventions. Microsatellite markers are useful genome features, which enable strain typing and identification in complex pathogen genomes. Here we describe microsatellite identification and characterization in T. solium, providing information that will assist in global efforts to control this important pathogen. METHODS: For genome sequencing, T. solium cysts and proglottids were collected from Huancayo and Puno in Peru, respectively. Using next generation sequencing (NGS) and de novo assembly, we assembled two draft genomes and one hybrid genome. Microsatellite sequences were identified and 36 of them were selected for further analysis. Twenty T. solium isolates were collected from Tumbes in the northern region, and twenty from Puno in the southern region of Peru. The size-polymorphism of the selected microsatellites was determined with multi-capillary electrophoresis. We analyzed the association between microsatellite polymorphism and the geographic origin of the samples. RESULTS: The predicted size of the hybrid (proglottid genome combined with cyst genome) T. solium genome was 111 MB with a GC content of 42.54%. A total of 7,979 contigs (>1,000 nt) were obtained. We identified 9,129 microsatellites in the Puno-proglottid genome and 9,936 in the Huancayo-cyst genome, with 5 or more repeats, ranging from mono- to hexa-nucleotide. Seven microsatellites were polymorphic and 29 were monomorphic within the analyzed isolates. T. solium tapeworms were classified into two genetic groups that correlated with the North/South geographic origin of the parasites. CONCLUSIONS/SIGNIFICANCE: The availability of draft genomes for T. solium represents a significant step towards the understanding the biology of the parasite. We report here a set of T. solium polymorphic microsatellite markers that appear promising for genetic epidemiology studies. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartof urn:issn:1935-2735
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Adult en_US
dc.subject Humans en_US
dc.subject Peru/epidemiology en_US
dc.subject Animals en_US
dc.subject Genetic Variation en_US
dc.subject Genotype en_US
dc.subject Geography en_US
dc.subject Microsatellite Repeats/genetics en_US
dc.subject Taenia solium/genetics/isolation & purification en_US
dc.subject Sequence Analysis, DNA en_US
dc.subject Base Sequence en_US
dc.subject Molecular Sequence Data en_US
dc.subject Chromosome Mapping en_US
dc.subject DNA, Helminth/chemistry/genetics en_US
dc.subject Genome, Helminth/genetics en_US
dc.subject High-Throughput Nucleotide Sequencing en_US
dc.subject Taeniasis/epidemiology/parasitology en_US
dc.title Identification and Characterization of Microsatellite Markers Derived from the Whole Genome Analysis of Taenia solium en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0004316
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE

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