Molecular Characterization of Extended-Spectrum beta-Lactamase-Producer Klebsiella pneumoniae Isolates Causing Neonatal Sepsis in Peru

Rojo-Bezares, Beatriz; Jacobs, Jan; Saenz, Yolanda; García Apac, Coralith Marlinda; Astocondor, Lizeth

dc.contributor.author	García Apac, Coralith Marlinda
dc.contributor.author	Astocondor, Lizeth
dc.contributor.author	Rojo-Bezares, Beatriz
dc.contributor.author	Jacobs, Jan
dc.contributor.author	Saenz, Yolanda
dc.date.accessioned	2019-02-06T14:57:36Z
dc.date.available	2019-02-06T14:57:36Z
dc.date.issued	2016
dc.identifier.uri	https://hdl.handle.net/20.500.12866/5460
dc.description.abstract	Klebsiella pneumoniae (KP) is the most common cause of neonatal sepsis in the low- and middle-income countries. Our objective was to describe the phenotypic and molecular characteristics of extended-spectrum beta-lactamase (ESBL)-producer KP in neonatal care centers from Peru. We collected 176 non-duplicate consecutive KP isolates from blood isolates of neonates from eight general public hospitals of Lima, Peru. The overall rate of ESBL production was 73.3% (N = 129). The resistance rates were higher among ESBL-producer isolates when compared with the nonproducers: 85.3% versus 12.8% for gentamicin (P < 0.01), 59.7% versus 8.5% for trimethoprim-sulfamethoxazole (P < 0.01), 45.0% versus 8.5% for ciprofloxacin (P < 0.01), and 36.4% versus 12.8% for amikacin (P < 0.01). A total of 359 beta-lactamase-encoding genes were detected among 129 ESBL-producer isolates; 109 isolates (84.5%) carried two or more genes. Among 37 ESBL-producer isolates randomly selected, CTX-M-15 and CTX-M-2 were the most common ESBLs detected. Most of the isolates (92%) belonged to the group KpI. Pulsed-field gel electrophoresis showed that multiple KP clones were circulating among the eight neonatal units included.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Humans	en_US
dc.subject	Peru/epidemiology	en_US
dc.subject	Drug Resistance, Bacterial	en_US
dc.subject	Gene Expression Regulation, Bacterial	en_US
dc.subject	Infant, Newborn	en_US
dc.subject	Anti-Bacterial Agents/pharmacology	en_US
dc.subject	beta-Lactamases/genetics/metabolism	en_US
dc.subject	Glucose Oxidase	en_US
dc.subject	Infant, Newborn, Diseases/epidemiology/microbiology	en_US
dc.subject	Klebsiella Infections/epidemiology/microbiology	en_US
dc.subject	Klebsiella pneumoniae/drug effects/enzymology/isolation & purification	en_US
dc.subject	Lactoperoxidase	en_US
dc.subject	Sepsis/epidemiology/microbiology	en_US
dc.title	Molecular Characterization of Extended-Spectrum beta-Lactamase-Producer Klebsiella pneumoniae Isolates Causing Neonatal Sepsis in Peru	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.15-0373
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645