Genome-Scale Protein Microarray Comparison of Human Antibody Responses in Plasmodium vivax Relapse and Reinfection

Vinetz, Joseph Michael; Brouwer, Kimberly C.; Gilman, Robert Hugh; Chuquiyauri, Raul; Gardner, Malcolm J.; Neafsey, Daniel E.; Torres, Sonia; Llanos Cuentas, Elmer Alejandro; Moss, Eli L.; Liang, Xiaowu; Wang, Ruobing; Felgner, Philip; Molina, Douglas M.

dc.contributor.author	Chuquiyauri, Raul
dc.contributor.author	Molina, Douglas M.
dc.contributor.author	Moss, Eli L.
dc.contributor.author	Wang, Ruobing
dc.contributor.author	Gardner, Malcolm J.
dc.contributor.author	Brouwer, Kimberly C.
dc.contributor.author	Torres, Sonia
dc.contributor.author	Gilman, Robert Hugh
dc.contributor.author	Llanos Cuentas, Elmer Alejandro
dc.contributor.author	Neafsey, Daniel E.
dc.contributor.author	Felgner, Philip
dc.contributor.author	Liang, Xiaowu
dc.contributor.author	Vinetz, Joseph Michael
dc.date.accessioned	2019-02-06T14:57:38Z
dc.date.available	2019-02-06T14:57:38Z
dc.date.issued	2015
dc.identifier.uri	https://hdl.handle.net/20.500.12866/5474
dc.description.abstract	Large scale antibody responses in Plasmodium vivax malaria remains unexplored in the endemic setting. Protein microarray analysis of asexual-stage P. vivax was used to identify antigens recognized in sera from residents of hypoendemic Peruvian Amazon. Over 24 months, of 106 participants, 91 had two symptomatic P. vivax malaria episodes, 11 had three episodes, 3 had four episodes, and 1 had five episodes. Plasmodium vivax relapse was distinguished from reinfection by a merozoite surface protein-3alpha restriction fragment length polymorphism polymerase chain reaction (MSP3alpha PCR-RFLP) assay. Notably, P. vivax reinfection subjects did not have higher reactivity to the entire set of recognized P. vivax blood-stage antigens than relapse subjects, regardless of the number of malaria episodes. The most highly recognized P. vivax proteins were MSP 4, 7, 8, and 10 (PVX_003775, PVX_082650, PVX_097625, and PVX_114145); sexual-stage antigen s16 (PVX_000930); early transcribed membrane protein (PVX_090230); tryptophan-rich antigen (Pv-fam-a) (PVX_092995); apical merozoite antigen 1 (PVX_092275); and proteins of unknown function (PVX_081830, PVX_117680, PVX_118705, PVX_121935, PVX_097730, PVX_110935, PVX_115450, and PVX_082475). Genes encoding reactive proteins exhibited a significant enrichment of non-synonymous nucleotide variation, an observation suggesting immune selection. These data identify candidates for seroepidemiological tools to support malaria elimination efforts in P. vivax-endemic regions.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Adolescent	en_US
dc.subject	Adult	en_US
dc.subject	Female	en_US
dc.subject	Humans	en_US
dc.subject	Male	en_US
dc.subject	Young Adult	en_US
dc.subject	Child	en_US
dc.subject	Child, Preschool	en_US
dc.subject	Aged	en_US
dc.subject	Aged, 80 and over	en_US
dc.subject	Polymerase Chain Reaction	en_US
dc.subject	Recurrence	en_US
dc.subject	Polymorphism, Single Nucleotide/genetics	en_US
dc.subject	Antibodies, Protozoan/immunology	en_US
dc.subject	Antibody Formation	en_US
dc.subject	Antigens, Protozoan/immunology	en_US
dc.subject	Gene Expression/immunology	en_US
dc.subject	Malaria, Vivax/immunology	en_US
dc.subject	Plasmodium vivax/genetics/immunology	en_US
dc.subject	Protein Array Analysis	en_US
dc.title	Genome-Scale Protein Microarray Comparison of Human Antibody Responses in Plasmodium vivax Relapse and Reinfection	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.15-0232
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645