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Genome-Scale Protein Microarray Comparison of Human Antibody Responses in Plasmodium vivax Relapse and Reinfection

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dc.contributor.author Chuquiyauri, Raul
dc.contributor.author Molina, Douglas M.
dc.contributor.author Moss, Eli L.
dc.contributor.author Wang, Ruobing
dc.contributor.author Gardner, Malcolm J.
dc.contributor.author Brouwer, Kimberly C.
dc.contributor.author Torres, Sonia
dc.contributor.author Gilman, Robert H.
dc.contributor.author Llanos-Cuentas, Alejandro
dc.contributor.author Neafsey, Daniel E.
dc.contributor.author Felgner, Philip
dc.contributor.author Liang, Xiaowu
dc.contributor.author Vinetz, Joseph M.
dc.date.accessioned 2019-02-06T14:57:38Z
dc.date.available 2019-02-06T14:57:38Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/5474
dc.description.abstract Large scale antibody responses in Plasmodium vivax malaria remains unexplored in the endemic setting. Protein microarray analysis of asexual-stage P. vivax was used to identify antigens recognized in sera from residents of hypoendemic Peruvian Amazon. Over 24 months, of 106 participants, 91 had two symptomatic P. vivax malaria episodes, 11 had three episodes, 3 had four episodes, and 1 had five episodes. Plasmodium vivax relapse was distinguished from reinfection by a merozoite surface protein-3alpha restriction fragment length polymorphism polymerase chain reaction (MSP3alpha PCR-RFLP) assay. Notably, P. vivax reinfection subjects did not have higher reactivity to the entire set of recognized P. vivax blood-stage antigens than relapse subjects, regardless of the number of malaria episodes. The most highly recognized P. vivax proteins were MSP 4, 7, 8, and 10 (PVX_003775, PVX_082650, PVX_097625, and PVX_114145); sexual-stage antigen s16 (PVX_000930); early transcribed membrane protein (PVX_090230); tryptophan-rich antigen (Pv-fam-a) (PVX_092995); apical merozoite antigen 1 (PVX_092275); and proteins of unknown function (PVX_081830, PVX_117680, PVX_118705, PVX_121935, PVX_097730, PVX_110935, PVX_115450, and PVX_082475). Genes encoding reactive proteins exhibited a significant enrichment of non-synonymous nucleotide variation, an observation suggesting immune selection. These data identify candidates for seroepidemiological tools to support malaria elimination efforts in P. vivax-endemic regions. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartof urn:issn:1476-1645
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Adolescent en_US
dc.subject Adult en_US
dc.subject Female en_US
dc.subject Humans en_US
dc.subject Male en_US
dc.subject Young Adult en_US
dc.subject Child en_US
dc.subject Child, Preschool en_US
dc.subject Aged en_US
dc.subject Aged, 80 and over en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject Recurrence en_US
dc.subject Polymorphism, Single Nucleotide/genetics en_US
dc.subject Antibodies, Protozoan/immunology en_US
dc.subject Antibody Formation en_US
dc.subject Antigens, Protozoan/immunology en_US
dc.subject Gene Expression/immunology en_US
dc.subject Malaria, Vivax/immunology en_US
dc.subject Plasmodium vivax/genetics/immunology en_US
dc.subject Protein Array Analysis en_US
dc.title Genome-Scale Protein Microarray Comparison of Human Antibody Responses in Plasmodium vivax Relapse and Reinfection en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.15-0232
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06


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